同位素比质谱方法检测兴奋剂福美司坦  

Detection of Formestane with Isotope Ratio Mass Spectrometry

作　　者：杨瑞[1,2] 杨文宁[3] 胡玉玲[2] 王静竹[1] 刘欣[1] 邢延一[1] 徐友宣[1] 曹建民[2] 

机构地区：[1]国家体育总局反兴奋剂中心,北京100029 [2]北京体育大学 [3]北京中医药大学

出　　处：《中国运动医学杂志》2015年第6期583-587,共5页Chinese Journal of Sports Medicine

基　　金：国家体育总局重点研究领域攻关课题(编号:2013B003)

摘　　要：目的:建立对福美司坦药物来源的检测方法,并进行人体代谢的检测研究。方法:采用酶解、提取、高效液相色谱纯化等方法对尿样进行预处理,应用同位素比质谱仪测定相关组分的同位素比(13C/12C,δ值),建立检测方法。本实验中招募2名男性健康受试者,每位受试者口服福美司坦后收集尿样,应用所建立的检测方法对尿样进行分析,探讨福美司坦在人体内代谢过程中的检测规律。结果:建立的分析方法精密度及重现性RSD均小于1%。受试者尿样中福美斯坦的δ值在服药后迅速降低,并在27小时内保持在约为-31‰。内源性雄性激素代谢物雄酮androsterone和本胆烷醇酮etiocholanolone的δ值无显著性变化。结论:在兴奋剂检测中确定以福美司坦药物原形为目标化合物进行来源分析,建立了检测福美斯坦兴奋剂的同位素比质谱分析方法。Objective The aim of this study is to set up a method for detecting formestane. Method Two healthy young volunteers were recruited and each of them orally administered 100 mg single dose of formestane and then their urine samples were collected. The sample preparation was completed through hydrolysis,extraction,and purification. Isotopic ratio（^13C/^12 C,δ value）of the sample was determined. Result The relative standard deviation of the precision and reproducibility was less than 1%. The δ values of formestane in the urine decreased rapidly after administration,but remained at about-31‰ within 27 hours of administration.The δ values of the main metabolites of endogenous androgenic steroids（androsterone and etiocholanolone）remained unchanged. Conclusion The method introduced in this study can detect formestane effectively.

关 键 词：福美司坦 同位素比质谱 兴奋剂 

分 类 号：R927[医药卫生—药学] O657.63[理学—分析化学]