浆细胞样树突状细胞对频繁复发的生殖器疱疹的免疫作用  

Immunological effect of plasmacytoid dendritic cells on frequently recurrent genital herpes

作　　者：杨闰平[1] 李安信[2] 李晓鹏[1] 刘元林[1] 

机构地区：[1]海军总医院皮肤科,北京100048 [2]解放军第三〇二医院门诊部皮肤性病科,北京100039

出　　处：《传染病信息》2015年第3期145-148,共4页Infectious Disease Information

摘　　要：目的探索浆细胞样树突状细胞(plasmacytoid dendritic cells,p DCs)对频繁复发的生殖器疱疹的免疫作用。方法选取确诊复发性生殖器疱疹患者10例,于静息期、复发早期及后期抽取外周血,通过梯度离心法分离白细胞,流式细胞术检测p DCs百分比,白细胞体外原代培养,ELISA法检测血清以及白细胞培养上清液干扰素(interferon,IFN)α和IFNγ,并与对照组比较。结果生殖器疱疹复发后期外周血p DCs百分比相较对照组、静息期及复发早期显著减低(P均<0.05);患者血清IFNα水平高于对照组,复发早期显著升高,后期降低(P均<0.05);患者IFNγ水平在疾病各期呈依次升高,差异均有统计学意义,静息期略低于正常参考值,发病早期和后期均高于正常参考值。IFNα、单纯疱疹病毒(herpes simplex virus,HSV)-2和寡聚脱氧核苷酸(Cp G-oligodeoxynueleotide,Cp G ODN)均可诱导外周血白细胞产生IFNγ,患者IFNγ水平显著低于对照组(P均<0.05)。BDCA-2抗体可抑制HSV-2和Cp G ODN的诱导作用。结论 p DCs参与了复发性生殖器疱疹的免疫反应,频繁复发的生殖器疱疹存在Th1淋巴细胞对p DCs介导的免疫反应减低。Objective To explore the immunological effect of plasmacytoid dendritic cells（p DCs） on frequently recurrent genital herpes. Methods A total of 10 patients with frequently recurrent genital herpes were selected. Peripheral blood of the patients at quiescent stage, early stage of recurrence and late stage of recurrence was drawn to isolate white blood cells（WBC） by density gradient centrifugation method. Percentages of p DCs were detected by flow cytometry. ELISA was used to detect the interferon（IFN） α and IFN γ in sera and supernate of primary WBC culture. All the results were compared with those of normal controls. Results The percentages of p DCs of patients at late stage of recurrence were significantly lower than those of the controls and the patients at quiescent stage and early stage of recurrence（P〈0.05）. Serum level of IFN α of the patients, significantly higher than that of the controls, increased at early stage of recurrence, and then decreased at late stage of recurrence significantly（P〈0.05）. Serum level of IFN γ at quiescent stage, early stage of recurrence and late stage of recurrence increased gradually, and the differences among them were significant; as compared with the controls,serum level of IFN γ at quiescent stage were significantly lower, and the levels of IFN γ at early and late stage of recurrence were significantly higher. IFN α, herpes simplex virus（HSV）-2 and Cp G-oligodeoxynueleotide（Cp G-ODN） could induce cultured WBC to secret IFNγ, which of the patients was significantly lower than that of the controls（P〈0.05）. Anti-BDCA-2 antibody could inhibit the induction of IFNγ by HSV-2 and Cp G-ODN. Conclusions p DCs are involve in immunological mechanisms of recurrent genital herpes. T-helper-1lymphocytes in patients with recurrent genital herpes exhibit reduced immune reactions mediated by p DCs.

关 键 词：树突细胞 浆细胞 疱疹病毒科感染 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]