植物源抗病毒剂VFB-1防治烟草花叶病毒病研究  

Control effect of phytogenic VFB-Ⅰ against tobacco mosaic virus disease

作　　者：陈丕庭[1] 马树杰[1] 殷培军[1,2] 郑伟[1] 马志卿[1,3] 张兴[1,3] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心,陕西杨凌712100 [2]黄岛出入境检验检疫局,山东青岛266555 [3]陕西省生物农药工程技术研究中心,陕西杨凌712100

出　　处：《中国烟草学报》2015年第3期93-99,共7页Acta Tabacaria Sinica

基　　金：国家公益性行业(农业)科研专项(200903052)

摘　　要：以植物源抗病毒剂VFB为基础,经配方改造和工艺改进,优化出了植物源抗病毒剂VFB-1。为探究VFB-1对烟草花叶病的防治作用,本研究采用半叶枯斑法测定了VFB-1对TMV的体外钝化、初侵染和复制增殖抑制活性;采用盆栽法和田间小区试验评价了VFB-1对烟草花叶病毒病的预防及治疗效果。结果表明,VFB-1对烟草花叶病毒(TMV)具有明显的体外钝化作用,钝化30min后,抑制率达82.77%;对TMV的初侵染和复制增殖具有较好的抑制作用,24 h的抑制率分别达到69.80%和54.07%?盆栽药效试验中,VFB-1100倍稀释液的预防效果为70.23%,治疗效果为35.70%;田间药效试验表明,VFB-1对烟草花叶病毒病有较好的预防和治疗作用,其200倍稀释液药后21d的预防效果仍为40%以上,400倍液的预防效果明显高于VFB,且与病毒A(20%吗啉胍·乙铜WP)相当;其200倍液14d的治疗效果显著高于VFB(200倍稀释液)和病毒A(400倍稀释液)处理。可见,植物源抗病毒剂VFB-1对TMV具有良好的预防和治疗效果,开发前景良好?Effect of VFB-I on tobacco mosaic virus（TMV） was evaluated with anti-TMV activities tested in vitro by local lesion. Protection and treatment effi ciencies of VFB-I on TMV were evaluated by pot experiment and fi eld plot trials. Results showed that VFB displayed signifi cant inactivation on TMV with inhibition rate of 82.75% after mixing VFB with TMV for 0.5 h. Infection and replication of TMV to Nicotiana glutinosa were also inhibited by VFB-I, with inhibition rate of 69.80% and 54.06%, respectively. Pot experiment showed that protection effi ciency of VFB- Ⅰ against TMV disease was 70.24% and remedial effi ciency was 35.71% by spraying with VFB- Ⅰ diluent of 1：100. In fi eld trials, protection effi ciency of VFB- Ⅰ diluent of 1：400 on TMV disease was higher than that of VFB, but equivalent to that of 20% moroxydine＋cupric acetate WP at the same concentration. The remedial effect of VFB- Ⅰ（diluent of 1：200） was much stronger than that of 20% moroxydine＋cupric acetate WP（diluents of 1：400） and VFB（diluents of 1：200）. VFB- Ⅰ showed higher anti-TMV activity indicating that it is effective in controlling TMV disease.

关 键 词：VFB-1 烟草花叶病毒 抗病毒活性 烟草花叶病 植物源农药 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]