豆蔻酰佛波醇乙酯对人心房肌细胞肿胀激活的氯离子电流的调节作用  

Regulation of swelling-activated chloride current in human atrial myocytes by phorbol-12-myristate-13-acetate

作　　者：李业涛[1] 杜心灵[2] 罗旻[3] 

机构地区：[1]贵州省人民医院心外科,贵州贵阳550002 [2]华中科技大学同济医学院附属协和医院心外科,湖北武汉430022 [3]贵州省人民医院肿瘤科,贵州贵阳550002

出　　处：《中国现代医学杂志》2015年第19期17-20,共4页China Journal of Modern Medicine

基　　金：贵州省科技计划(No:黔科合LS[2011]040号)

摘　　要：目的通过全细胞膜片钳技术研究蛋白激酶C(PKC)激动剂豆蔻酰佛波醇乙酯(PMA)对人心房肌细胞肿胀激活的氯离子电流(ICl.swell)的作用。方法术中取患者右心房组织,酶解分离出心房肌细胞。全细胞膜片钳技术记录氯离子电流。低渗台氏液灌流激活胞肿胀激活的氯离子电流后再加入PMA,观察电流变化。结果胞肿胀激活的氯离子电流达到稳定状态后再用含有1μmol/L PMA的低渗台氏液灌流,从-110 m V到+60 m V,除了在-10 m V,电流密度均增加(P<0.05,n=7)。该效应在洗脱后或者加入氯离子电流阻断剂二异氰酸二磺酸后减弱。结论该研究通过全细胞膜片钳技术观察到蛋白激酶C激动剂PMA可以增强人心房肌细胞ICl.swell。但对其机制,还需要进一步研究。[Objective] To study the function of phorbol-12-myristate-13-acetate （PMA）, a protein kinase C （PKC） agortist, in the regulation of swelling-activated chloride currents （ICl.swell） in human atrial myocytes via the whole-cell patch-clamp technique. [Methods] Human right atria/appendages were obtained from patients undergo- ing cardiosurgery. Single human atrial myocytes were dispersed enzymatically. ICl.swell was evoked under hypotonic solution and recorded via the whole-cell patch-clamp technique, either in the presence or absence of PMA. [ Re- suits ] After ICl.swell approached steady state, subsequent exposure to 1 μmol/L PMA caused substantial augment of current density in the whole range of voltage （from -110 mV to ＋60 mV） except in -10 mV （P 〈 0.05, n = 7）. The effect attenuated after washout or the addition of chloride current inhibitor, 4,4-diisothiocyanostilbene -2,2-disulfon- ic acid. [ Conclusions ] By using the whole-cell patch-clamp technique, PMA, a PKC agonist, was found to enhance ICl.swell in human atrial myocytes, and the underlying mechanism needs further study.

关 键 词：肿胀激活的氯离子电流 豆蔻酰佛波醇乙酯/PMA 蛋白激酶C 人类 心房肌细胞 

分 类 号：Q331[生物学—遗传学] R541[医药卫生—心血管疾病]