热休克蛋白47在单侧输尿管梗阻大鼠肾小管间质中的表达变化  

Expression of HSP47 in kidneys of rats with unilateral ureteral obstruction

作　　者：毕慧欣[1] 刘瑞洪[1] 刘虹[2] 孙林[2] 袁曙光[2] 黄谷香[1] 

机构地区：[1]中南大学湘雅二医院老年病科,湖南长沙410011 [2]中南大学湘雅二医院肾内科,湖南长沙410011

出　　处：《中国现代医学杂志》2015年第20期32-38,共7页China Journal of Modern Medicine

摘　　要：目的建立单侧输尿管梗阻(UUO)大鼠肾小管上皮细胞转分化(EMT)模型,检测EMT过程中热休克蛋白47(HSP47)的表达变化。方法采用UUO模型,将雄性SD大鼠36只随机分为假手术组(Sham组)和模型组(UUO组),每组各18只。术后3、7和14 d分别处死两组大鼠。采用HE和MASSON染色法观察各组大鼠术后不同时间肾脏病理改变,用免疫组织化学检测HSP47、波形蛋白(Vimentin)及紧密连接蛋白(ZO-1)的表达。结果随着梗阻时间延长,UUO组大鼠肾小管损伤程度和肾间质纤维化程度逐渐增加,HSP47和Vimentin表达上升(P<0.05),而ZO-1表达下降(P<0.05)。结论 UUO模型大鼠EMT过程中伴有HSP47的高表达,HSP47表达上升可促进EMT过程。[Objectives] To detect the expression of HSP47 in the kidneys of rats with unilateral ureteral obstruction. [Methods] Male Sprague-Dawley （SD） rats were randomly divided into sham group （n = 18） and unilateral ureteral obstructive （UUO） model group （n= 18）. The obstructed kidneys were harvested 3, 7 and 14d after UUO. Changes in renal morphology were examined by HE and Masson staining. The expressions of HSP47, vimentin and zona occludens-1 （ZO-1） were examined by immunohistochemistry （IHC） in each group. [Results] Compared with the sham group, the expressions of HSP47 and vimentin were markedly upregulated in the UUO model group （P〈 0.05）, but the expression of ZO-1 was downregulated （P〈 0.05）, and more severe tubular injury and tubulointerstitial fibrosis were also noticed in the UUO model rats （P〈 0.05）. [Conclusions] HSP47 is upregulated in the progress of epithelial to mesenchymal transition （EMT） in the UUO model rats. HSP47 may accelerate this process.

关 键 词：肾间质纤维化 HSP47 上皮细胞间充质转分化 

分 类 号：R692.33[医药卫生—泌尿科学]