GC法同时测定艾纳香油中5个主要成分的含量  

Simultaneous determination of five components in Blumea balsamifera oil by GC

作　　者：吴丽芬[1,2,3] 庞玉新[1,3] 杨全[2] 袁蕾[1,3] 张影波 李海艳[1,2,3] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室,儋州571737 [2]广东药学院中药学院,广州510006 [3]海南省艾纳香工程技术研究中心,儋州571737

出　　处：《药物分析杂志》2015年第7期1179-1184,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(81374065);黔科合重大专项字([2013]6011)

摘　　要：目的：建立GC法同时测定艾纳香油中β-蒎烯、芳樟醇、樟脑、L-龙脑、β-石竹烯5个成分的含量。方法：采用HP-5毛细管柱（30 m×0.32 mm,0.25μm）,程序升温（初始温度70℃,保持2 min,以5℃·min-1升温至最终温度150℃,保持2min）,FID检测器温度240℃,进样口温度240℃,载气为高纯氮气,流速6.5 m L·min-1,进样量0.6μL,进样分流比19∶1。结果：β-蒎烯、芳樟醇、樟脑、L-龙脑、β-石竹烯质量浓度分别在0.062 7∽0.940 8 mg·m L-1（r=0.999 9）、0.031 4∽0.470 4mg·m L-1（r=0.999 9）、0.141 8∽2.127 2 mg·m L-1（r=0.999 9）、0.329 7∽4.946 0 mg·m L-1（r=0.999 9）、0.178 8∽1.788 7mg·m L-1（r=0.999 9）范围内具有良好线性关系;平均回收率（n=9）分别为99.5%,99.7%,99.9%,99.7%,100.1%;各批次艾纳香油中β-蒎烯、芳樟醇、樟脑、L-龙脑、β-石竹烯5个主要成分的含量范围分别为27.3∽56.5、16.1∽23.0、83.6∽186.7、192.3∽255.3和82.8∽185.1 mg·g-1。结论：该方法符合方法学验证要求,操作简便、准确,精密度、分离度良好,分析时间短,可用于艾纳香油的多成分含量测定及质量控制。Objective ：To establish a GC method for simultaneous determination of β-pinene, linalool, camphor, l- borneol and β-caryophyllene in Blurnea balsamifera oil. Methods： The GC analysis was carried out on an HP-5 capillary column （ 30 m ×. 32 mm, 0. 25μm ） with the temperature program as follows ： 70 ℃ for 2 rain, 5 ℃ min-1 to 150 ℃ ,retaining 2min . The FID detector was controlled at 240 9C. The inlet temperature was set at 240 ℃. The carrying gas was high-purity nitrogen（6. 5 mL· min-1 ） ,split ration was 19：1 ,and the injection volume was 0. 6μL. Results： The methodology validation for the assay of fl-pinene, linalool, camphor, l-borneol and β-caryophyllene presented that they were in good linear correlation in the ranges of 0. 062 7-0. 940 8 mg· mL-1（r =0. 999 9） ,0. 031 4-0.470 4 mg·mL-1 （r =0. 999 9） ,0. 141 8-2. 127 2 mg·mL-1 （r =0.999 9） ,0. 329 7-4. 946 0mg· mL-1 （r =0. 999 9） ,and 0. 178 8-1. 788 7 mg·mL-l（r =0. 999 9） ,with the average recoveries （ n = 9） of 99.5 % ,99.7 % ,99.9% ,99.7 % , and 100. 1%. In all batches of Blumea balsamifera oil, the ranges of five components were 27.3-56. 5 mg·g-1 for β-pinene, 16. 1-23.0 mg· g-1 for linalool,83.6-186. 7 -1 -1 mg·g for camphor, 192. 3-255.3 mg· g for -borneol and 82. 8-185.1 mg · g-l for β-caryophyllene, respec- tively. Conclusion： The established method is in accordance with the requirements of the methodology validation, which is simple, quick and accurate. It can be used for simultaneous determination of multiple components and the quality control of Blumea balsamifera oil.

关 键 词：艾纳香油 Β-蒎烯 芳樟醇 樟脑 L-龙脑 β-石竹烯 气相色谱法 

分 类 号：R917[医药卫生—药物分析学]