川芎嗪注射液对Jurkat细胞增殖、凋亡的影响及其相关机制  

Effects of tetramethylpyrazine injection on the proliferation and apoptosis of Jurkat cells and the relevant molecular mechanisms

作　　者：王建勇[1] 贾秀红[1] 李娜[1] 伊英杰[1] 王红[1] 

机构地区：[1]滨州医学院附属医院儿科,256603

出　　处：《白血病．淋巴瘤》2015年第6期341-345,共5页Journal of Leukemia & Lymphoma

基　　金：山东省自然科学基金（ZR2014HL032）;山东省医药卫生科技发展计划（2014WS0183）;滨州医学院科技计划（BY2011KJ033）

摘　　要：目的 探讨川芎嗪注射液对人类急性淋巴细胞白血病（ALL） Jurkat细胞株增殖与凋亡的影响,并进一步研究其相关分子机制.方法 用质量浓度分别为0、0.25、0.50、0.75、1.00、1.25、1.50 mg/ml的川芎嗪注射液作用Jurkat细胞24、48、72 h后,CCK-8法检测细胞增殖的变化;质量浓度为0、0.50、1.00、1.50 mg/ml川芎嗪注射液处理Jurkat细胞48 h后,流式细胞术检测细胞周期与凋亡的变化,Western blot检测细胞Aurora-B及Survivin蛋白的表达.结果 与未经川芎嗪注射液处理细胞组（对照组）相比,不同浓度川芎嗪注射液能有效抑制Jurkat细胞的增殖,并呈剂量和时间依赖性（P＜0.05）,24、48及72 h的IC50分别为（1.33±0.16）、（0.91±0.10）、（0.67±0.11）mg/ml.质量浓度0.5、1.0、1.5 mng/ml的川芎嗪注射液作用Jurkat细胞48 h后,与对照组相比,G2/M期细胞比例增高,S期细胞比例降低,细胞凋亡率升高,并呈剂量依赖性（P＜0.05）;Western blot结果显示,川芎嗪注射液以浓度依赖方式下调Aurora-B和Survivin蛋白表达（P＜0.05）.结论 川芎嗪注射液在体外能有效抑制Jurkat细胞的增殖并诱导其凋亡,其机制可能与下调Aurora-B、Survivin蛋白的表达相关.Objective To study the effect of tetramethylpyrazine injection on proliferation and apoptosis of human acute lymphoblastic leukemia （ALL） cell line Jurkat and the relevant molecular mechanisms.Methods Cells were treated with tetramethylpyrazine injection at various concentrations （0,0.25,0.50,0.75,1.00,1.25 and 1.50 mg/ml）,CCK-8 method was used to detect the inhibition rates at 24,48 and 72 h.After cells were treated with different concentrations of tetramethylpyrazine injection （0,0.50,1.00 and 1.50 mg/ml） for 48 h,the cell cycle and apoptosis were detected by flow cytometry,and the Aurora-B and Survivin protein expression were analyzed by Western blot.Results Compared with the control group （cells without tetramethylpyrazine injection treatment）,various concentrations of tetramethylpyrazine injection could effectively inhibit Jurkat cells proliferation in a time-and dose-dependent manner （P 〈 0.05）,and IC50 at 24 h,48 h and 72 h after treatment were （1.33±0.16）,（0.91±0.10） and （0.67±0.11） mg/ml,respectively.After cells were treated with tetramethylpyrazine injection at different concentrations （0.5,1.0 and 1.5 mg/ml） for 48 h,the number of treated cells in G2/M phase was increased,but that in S phase was decreased,and the apoptosis rates were significantly higher than that of control group,with a dose dependence （P 〈 0.05）.The results of Western blot showed that the expression levels of Aurora-B and Survivin in treated cells were lower than that of control group,also with a dose dependence （P 〈 0.05）.Conclusions Tetramethylpyrazine injection can effectively inhibit proliferation and induce apoptosis of Jurkat cells in vitro,and its underlying mechanisms involve with down-regulation of the Aurora-B and Survivin protein expression.

关 键 词：川芎嗪注射液 白血病 细胞增殖 细胞凋亡 

分 类 号：R96[医药卫生—药理学]