HPLC-DAD分析金线莲中天麻素和天麻苷元  

Analysis of Gastrodine and 4-hydroxybenzyl Alcohol in A. roxburghii by HPLC-DAD

作　　者：陈惠芳 吴萍萍[2] 黄丽英[2] 陈瑜[3] 林守二 

机构地区：[1]泉州医学高等专科学校,福建泉州362000 [2]福建医科大学药学院,福建福州350004 [3]福建医科大学基础医学院,福建福州350004

出　　处：《分析测试技术与仪器》2015年第2期69-76,共8页Analysis and Testing Technology and Instruments

基　　金：国家卫生和计划生育委员会科研基金课题(wkj-FJ-33);福建省自然基金项目(2012J01363)

摘　　要：研究超声-微波协同法提取金线莲中的天麻素和天麻苷元,并对提取条件进行了优化.应用高效液相-二极管阵列检测法分析提取物中的天麻素和天麻苷元.研究结果表明:金线莲中天麻素、天麻苷元和其他杂质成分能够很好的分离,且两者的含量分别为1.430及0.562 mg/g,相对标准偏差为0.94%和0.25%.方法准确、快速、灵敏度高、重现性好.The ultrasound-microwave assisted extraction （ UMAE ） method has been applied to the extraction of Anoectocilus roxburghii（ A. roxburghii） and the extraction conditions were optimized in this study. A method of HPLC-DAD was developed for the simultaneous identification and determination of gastrodine and 4-hydroxybenzyl alcohol. Results showed that the contents of gastrodine and 4-hydroxybenzyl alcohol from A. roxburghii were 1. 430 mg/g and 0. 562 mg/g, and relative standard deviations were 0. 94% and 0. 25% respectively. The proposed method is simple, accurate, rapid and reproducible.

关 键 词：金线莲 天麻素 天麻苷元 超声-微波提取 高效液相-二极管阵列检测法 

分 类 号：O657.7[理学—分析化学]