Biphasic Mechanical Properties of in vivo Repaired Cartilage  

作　　者：Qin Lian Cheng Chen Marie Chantal Uwayezu Weijie Zhang Weiguo Bian Junzhong Wang Zhongmin Jin 

机构地区：[1]State Key Laboratory for Manufacturing Systems Engineering, Xi 'an Jiaotong University, Xi 'an 710054, China [2]Orthopaedics Department, First Affiliated Hospital, Xi 'an Jiaotong University, Xi 'an 710061, China [3]Institute of Medical and Biological Engineering, School of Meehanical Engineering, University of Leeds, Leeds LS2 9JT, UK

出　　处：《Journal of Bionic Engineering》2015年第3期473-482,共10页仿生工程学报（英文版）

基　　金：This work was supported by grants from the Native Science Foundation of China （Nos. 51323007, 51375371 and 51075320）, the National High Technology Research and Development Program of China （No. 2015AA020303） and the Fundamental Research Funds for the Central Universities. The authors would like to acknowledge the contributions of Dichen Li, Manyi Wang, Yongmei Chen and Yusheng Qiu of Xi＇an Jiaotong University.

摘　　要：In the fast growing field of scaffold-based tissue engineering, improvement on the mechanical properties of newly formed tissues, e.g. the repaired cartilage, has always been one of the core issues. Studies on the correlations among scaffold composition, in vivo morphological changes of the construct, and the finite deformation behaviors of new tissues （e.g. creep and stress-relaxation, and equilibrium response）, have attracted increasing interests. In this paper, the correlations between the compressive biphasic mechanical properties （i.e., equilibrium elastic modulus E and permeability coefficient k） of 3D printing scaffold （consisting of collagen and fl-tricalcium phosphate） and the proteoglycans （PGs） concentration of the repaired carti- lages after 24 weeks, 36 weeks and 52 weeks of scaffold implantation were investigated. Results indicated that the repaired cartilage covered the entire cartilage surface of large cylindrical osteochondral defects （10 mm in diameter ~ 15 mm in depth） on the canine trochlea grooves after 24 weeks. The equilibrium elastic modulus of the repaired cartilage reached 22.4% at 24 weeks, 70.3% at 36 weeks, and 93.4% at 52 weeks of the native cartilage, respectively. Meanwhile, the permeability coefficient decreased with time and at 52 weeks was still inferior to that of the native cartilage in one order of magnitude. In addition, the amount of glycosaminoglycans （GAGs） of repaired cartilage increased constantly with time, which at 52 weeks approached to nearly 60% of that of native cartilage. 3D printed scaffolds have potential applications in repairing large-scale cartilage defects.In the fast growing field of scaffold-based tissue engineering, improvement on the mechanical properties of newly formed tissues, e.g. the repaired cartilage, has always been one of the core issues. Studies on the correlations among scaffold composition, in vivo morphological changes of the construct, and the finite deformation behaviors of new tissues （e.g. creep and stress-relaxation, and equilibrium response）, have attracted increasing interests. In this paper, the correlations between the compressive biphasic mechanical properties （i.e., equilibrium elastic modulus E and permeability coefficient k） of 3D printing scaffold （consisting of collagen and fl-tricalcium phosphate） and the proteoglycans （PGs） concentration of the repaired carti- lages after 24 weeks, 36 weeks and 52 weeks of scaffold implantation were investigated. Results indicated that the repaired cartilage covered the entire cartilage surface of large cylindrical osteochondral defects （10 mm in diameter ~ 15 mm in depth） on the canine trochlea grooves after 24 weeks. The equilibrium elastic modulus of the repaired cartilage reached 22.4% at 24 weeks, 70.3% at 36 weeks, and 93.4% at 52 weeks of the native cartilage, respectively. Meanwhile, the permeability coefficient decreased with time and at 52 weeks was still inferior to that of the native cartilage in one order of magnitude. In addition, the amount of glycosaminoglycans （GAGs） of repaired cartilage increased constantly with time, which at 52 weeks approached to nearly 60% of that of native cartilage. 3D printed scaffolds have potential applications in repairing large-scale cartilage defects.

关 键 词：biphasic mechanical properties PGS repaired cartilage osteochondral scaffolds 3D printing 

分 类 号：TG146.21[一般工业技术—材料科学与工程] TS251.94[金属学及工艺—金属材料]