造纸法再造烟叶加工过程中5种甾醇的含量变化  

Change of Sterol Contents in Paper-making Process Reconstituted Tobacco During Processing

作　　者：孙德坡 王昆淼 赵伟 申钦鹏 何沛 刘东辉[2] 唐自文 刘恩芬 刘志华 陈永宽 

机构地区：[1]云南中烟工业有限责任公司技术中心云南省烟草化学重点实验室,昆明市五华区红锦路367号650231 [2]昆明理工大学化学工程学院 [3]云南中烟再造烟叶有限责任公司,昆明市高新区科医路41号650106

出　　处：《烟草科技》2015年第7期59-63,共5页Tobacco Science & Technology

基　　金：中国烟草总公司科技重大专项项目"选择性降低烟草中苯并[a]芘前体化合物减害配方技术研究"[110201101030(JH-05)]和"具有降低苯并[a]芘功能的造纸法再造烟叶研究及应用"[110201301025(JH-06)]

摘　　要：为研究造纸法再造烟叶各工艺阶段甾醇类化合物的含量变化,利用超高效液相色谱-串联质谱仪(UPLC-MS/MS)对5种甾醇(麦角甾醇、胆甾醇、菜油甾醇、豆甾醇及β-谷甾醇)进行了测定。结果表明:1烟叶原料中5种甾醇的含量(质量分数)均高于烟梗,且两种烟草原料中的甾醇类化合物均以豆甾醇和β-谷甾醇为主;2在提取和浓缩阶段,甾醇含量均降低,降低量分别占原料中甾醇总量的21.35%和15.13%;3在掺配阶段,低浓浆中甾醇实际增加量占原料中甾醇总量的10.64%;4在打浆和抄造阶段,甾醇含量均降低,降低量分别占原料中甾醇总量的14.06%和14.18%;5其余工艺阶段的甾醇含量变化不大,最终再造烟叶产品中甾醇量占原料中甾醇总量的48.67%。In order to investigate the change of sterol contents in paper-making process reconstituted tobacco at various processing stages, the contents of five sterols （ergosterol, cholesterol, campesterol, stigmasterol and β-sitosterol） were determined by ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. The results showed that： 1） The contents of the said sterols were higher in tobacco leaves than in tobacco stems, and the major sterols in tobacco leaves and stems were stigmasterol and/3-sitosterol. 2） The content of sterols decreased by 21.35% and 15.13% at extraction and concentration stages, respectively. 3） At blending stage, the content of sterols in low consistency pulp actually increased by 10.64%. 4） At beating and papermaking stages, the content of sterols decreased by 14.06% and 14.18%, respectively. 5） Sterol content changed little at the other processing stages. The sterol content remaining in finished reconstituted tobacco was 48.67% of that in raw material.

关 键 词：纸法再造烟叶 加工过程 甾醇 超高效液相色谱-串联质谱仪(UPLC-MS/MS) 

分 类 号：TS411.1[农业科学—烟草工业] TS452.6