蒙药复方森登-4体内外化学成分分析  

Analyzing chemical composition of Mongolian compound Sengdeng-4 in vivo and in vitro

作　　者：萨础拉[1] 白埔 董玉[1] 邢凯霞 

机构地区：[1]内蒙古医科大学,内蒙古自治区010110

出　　处：《北京中医药大学学报》2015年第6期400-404,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81160550);内蒙古自治区自然科学基金资助项目(No.2013JQ03);内蒙古医科大学"英才培育"资助项目

摘　　要：目的建立蒙药复方森登-4体内外特征图谱,归属并鉴定其体外化学成分及体内吸收入血成分。方法取SD大鼠,建立类风湿性关节炎动物模型,灌胃蒙药复方森登-4及其各单味药有效组分,分离制备含药血清,HPLC法测定,建立蒙药复方森登-4体外及含药血清HPLC特征图谱,分析蒙药复方森登-4、各单味药体外及其大鼠含药血清特征图谱,比对、归属并鉴定蒙药复方森登-4体外化学成分及口服蒙药复方森登-4后的大鼠吸收入血成分。结果分析蒙药复方森登-4体外HPLC特征图谱,发现了共47个峰;蒙药复方森登-4灌胃给药后出现了14个入血成分,9个为体外成分直接入血,另外5个推测为新生代谢产物,2号峰来源于文冠木,5、6、7号峰来源于栀子,1、8、10号峰来源于诃子,3号峰来源复杂,均来源于文冠木、川楝子、诃子,4、13号峰来源于全方,9号峰来源于文冠木及川楝子,11、12、14号峰来源于文冠木及诃子并鉴定出7个蒙药复方森登-4成分。结论血中成分及代谢产物可能为蒙药复方森登-4体内直接作用的有效成分,分别来源于蒙药复方森登-4的各单味药。Objective To establish the characteristic chromatograms of Mongolian compound Sengdeng-4in vivo and in vitro,analyze and identify its chemical components in vitro and serum. Methods After the model of rheumatoid arthritis was established,SD rats were orally administered Mongolian compound Sengdeng-4 and each single ingredient respectively. The medicinal serum were separated and measured with HPLC. The HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 in vitro and medicinal serum were identified. The characteristic chromatograms of Mongolian compound Sengdeng-4,single ingredient in vitro and medicinal serum were analyzed,and the chemical composition of Mongolian compound Sengdeng-4 in vitro, original crude medicinal in blood were identified and compared.Results The results of HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 showed that 47 peaks were identified. There were 14 chemical compositions absorbed into blood,among them 9were original ingredients and 5 were metabolites. The second peak was from Wenguanmu; the fifth,sixth,and seventh peaks were from Zhizi; the first,eighth,and tenth peaks were from Hezi. The third peak was from Wenguanmu,Chuanlianzi and Hezi. The fourth and thirteenth peaks were from Mongolian compound Sengdeng-4. The ninth peak was from Wenguanmu and Chuanlianzi. The eleventh,twelfth and fourteenth peaks were from Wenguanmu and Hezi. 7 components of Mongolian compound Sengdeng-4were identified. Conclusion The chemical compositions and metabolites in blood may be the active ingredients with direct actions in vivo, which come from the ingredients of Mongolian compound Sengdeng-4.

关 键 词：蒙药森登-4 特征图谱 化学成分 血清 

分 类 号：R284.1[医药卫生—中药学]