盐酸吗啉胍片对小鼠心肌细胞的毒性研究  

The toxic effects of Moroxydine Hydrochloride Tablets on mice myocardial cells

作　　者：郝琦[1,2] 华菲[1,2] 宋凡[1,2] 张环[3] 王四旺[1,2] 谢艳华[1,2] 孙纪元[1,2] 

机构地区：[1]第四军医大学药学系天然药物学教研室,西安710032 [2]秦巴山区中药发展协同创新中心,西安710032 [3]西安市中心医院心血管内科,西安710003

出　　处：《西北药学杂志》2015年第4期379-382,共4页Northwest Pharmaceutical Journal

摘　　要：目的探究盐酸吗啉胍片(ABOB)对心肌细胞的毒性。方法将小鼠随机分为盐酸吗啉胍片高剂量组(8只)、盐酸吗啉胍片低剂量组(8只)、生理盐水对照组(8只),给小鼠灌胃给药7d后,检测小鼠血清中肌酸激酶(CK)、脑钠肽(BNP)、肌钙蛋白T(CTnT)的水平;HE染色对心脏、肾脏的病理学形态分析。同时对新生大鼠心肌细胞培养,用MTT法检测盐酸吗啉胍片对心肌细胞的抑制作用。结果与对照组比较,盐酸吗啉胍片高剂量组、盐酸吗啉胍片低剂量组的CK水平增高,差异有统计学意义(P<0.05),BNP和CTnT水平差异无统计学意义;与对照组比较,盐酸吗啉胍片高剂量组、盐酸吗啉胍片低剂量组的心肌组织切片呈病理样改变,肾脏组织未见明显变化;与空白对照组比较,给药组心肌细胞活性受到不同程度的抑制,并随着给药剂量的增加,心肌细胞活性逐渐下降。结论盐酸吗啉胍片对心肌细胞有一定的毒性。Objective To explore the myocardial cell toxicity of Moroxydine Hydrochloride Tablets（ABOB）.Methods Mice were randomly divided into ABOB high dose group（8）,low dose group（8）,and saline control group（8）.Mice serum creatine kinase（CK）,brain natriuretic peptide（BNP）,CTnT levels were determined 7dafter intragastric administration.The hearts and kidneys were removed and processed for histological examination.At the same time,the inhibition effect of ABOB on the newborn rat myocardial cell was evaluated by MTT assay.Results Compared with the control group,ABOB high dose group,ABOB low dose group significantly increased the levels of CK（P〈0.05）,while no obvious change was observed in the levels of BNP and CTnT.Compared with the control group,ABOB high dose group and ABOB low dose group,myocardial tissue presented patholoical change,while no obvious change found in kidney tissue.Compared with the control group,myocardial cell activity was inhibited in different degrees after administration of ABOB with high or low dose,and tended to decline gradually with increasing the drug dose.Conclusion ABOB has certain toxicity for myocardial cells.

关 键 词：盐酸吗啉胍片 肌酸激酶 脑钠肽 肌钙蛋白T 心肌细胞 

分 类 号：R965[医药卫生—药理学]