HPLC法测定马钱子碱纳米结构脂质载体的主药含量及包封率  

Determination of the Main Content and Entrapment Efficiency in the Brucine Nanostructured Lipid Carrier by HPLC

作　　者：管庆霞[1] 华晓丹 张喜武[1] 李秋红[1] 李伟男[1] 赵宇巍[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《中国药房》2015年第21期2983-2986,共4页China Pharmacy

基　　金：黑龙江省教育厅科学技术研究项目(No.12531624)

摘　　要：目的：建立测定马钱子碱纳米结构脂质载体中主药含量及包封率的方法。方法：采用高效液相色谱法测定主药含量,测定包封率时以葡聚糖凝胶柱过滤法分离马钱子碱纳米结构脂质载体中的游离药物。色谱柱为Dikma C18,流动相为流动相A（甲醇）-流动相B[水-乙酸-三乙胺（230∶2.4∶0.3,V/V/V）]（30∶70,V/V）,流速为1 ml/min,检测波长为265 nm,进样量为10μl,柱温为30℃。结果：马钱子碱质量浓度在4.00~80.00μg/ml范围内与其峰面积呈良好的线性关系（r=0.999 9）;精密度、稳定性、重复性试验的RSD≤1.67%;含量测定平均加样回收率为99.66%,RSD=0.45%（n=9）;葡聚糖凝胶柱过滤法平均回收率为99.75%,RSD=1.74%（n=9）;平均包封率为69.92%。结论：该方法操作方便、重复性好、效率高,可用于马钱子碱纳米结构脂质载体中的主药含量及包封率的测定。OBJECTIVE：To establish a method for determining the main contents and entrapment efficiency in the brucine nanostructured lipid carrier（NLC）. METHODS：HPLC was adopted to determine the main content,sephadex gel filtration method was employed to separate free drug in brucine NLC to determine the entrapment efficiency. The column was Dikma C18 with the mobile phase of mobile phase A（methanol）-mobile phase B [water-acetic acid-triethylamine（230 ∶ 2.4 ∶ 0.3,V/V/V）]（30 ∶ 70,V/V）at the flow rate of 1 ml/min,the detection wavelength was 265 nm,volume was 10 μl and temperature was 30 ℃. RESULTS：The linear range of brucine was 4.00-80.00 μg/ml（r=0.999 9）;RSDs of precision,stability and reproducibility tests were ≤1.67%;average recoveries of content determination and sephadex gel filtration method were respectively 99.66%（RSD=0.45%,n=9） and99.75%（RSD=1.74%,n=9）;and the average entrapment efficiency was 69.92%. CONCLUSIONS：The method is simple,reproducible and efficient,and can be used for the determination of main contents and entrapment efficiency in brucine NLC.

关 键 词：马钱子碱 纳米结构脂质载体 高效液相色谱法 葡聚糖凝胶柱过滤法 含量 包封率 

分 类 号：R917[医药卫生—药物分析学] R943[医药卫生—药学]