HPLC法测定罗浮山风湿膏药中盐酸麻黄碱的含量  

Content Determination of Ephedrine Hydrochloride in Luofu Mountain Rheumatism Plaster by HPLC

作　　者：杨家彬[1] 张先平[2] 吴世平[1] 龚少智[1] 

机构地区：[1]荆州市第一人民医院皮肤科,湖北荆州434000 [2]荆州市第一人民医院药学部,湖北荆州434000

出　　处：《中国药房》2015年第21期2994-2996,共3页China Pharmacy

摘　　要：目的：建立测定罗浮山风湿膏药中盐酸麻黄碱含量的方法。方法：采用高效液相色谱法。色谱柱为Eclipse XDB-C18,流动相为乙腈-0.1%磷酸溶液（9∶91,V/V）,流速为1.0 ml/min,检测波长为207 nm,柱温为40℃,进样量为5μl。结果：盐酸麻黄碱进样量在81.6~408 ng范围内与其峰面积呈良好的线性关系（r=0.999 7）,精密度、稳定性、重复性试验的RSD均〈2%,平均加样回收率为96.73%,RSD为1.9%（n=6）。结论：该方法快速、准确,适用于罗浮山风湿膏药中盐酸麻黄碱的含量测定。OBJECTIVE：To establish a method for the content determination of ephedrine hydrochloride in Luofu mountain rheumatism plaster. METHODS：HPLC was performed on the column of Eclipse XDB-C18 with the mobile phase of acetonitrile-0.1% phosphoric acid（9 ∶ 91,V/V）,at the flow rate of 1.0 ml/min. The detection wavelength was 207 nm,the column temperature was 40 ℃,and the volume was 5 μl. RESULTS：The linear range of ephedrine hydrochloride was 81.6-408 ng（r=0.999 7）;RSDs of precision,stability and repeatability tests were lower than 2%;and the average recovery rate was 96.73%（RSD=1.9%,n=6）. CONCLUSIONS：The method is fast and accurate,and can be used for the content determination of ephedrine hydrochloride in Luofu mountain rheumatism plaster.

关 键 词：罗浮山风湿膏药 盐酸麻黄碱 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]