去势后阻断自噬对大鼠前列腺细胞凋亡的影响  

The influence of blocking autophagy to apoptosis in rat prostate after castration

作　　者：凌生涛[1] 姚启盛[1] 柳建军[2] 陈从波[1] 黄力[1] 王黎[1] 

机构地区：[1]十堰湖北医药学院附属太和医院泌尿外科,湖北442000 [2]湛江广东医学院附属医院泌尿外科,广东524000

出　　处：《国际泌尿系统杂志》2015年第4期527-530,共4页International Journal of Urology and Nephrology

摘　　要：目的探讨去势后阻断自噬对大鼠前列腺细胞凋亡的影响。方法sD雄性大鼠72只随机分为3组：即假切对照组、去势组、去势＋硫酸羟氯喹处理组。各组大鼠分别于术后第1、3、7、10、14、21d随机抽取4只，完整取出前列腺组织。HE染色观察大鼠前列腺组织形态学变化，电镜下观察前列腺微观结构、自噬和凋亡的变化，免疫组化SP法检测自噬受体蛋白P62和凋亡促进蛋白Caspase-3在前列腺组织中表达。结果光镜下去势后大鼠前列腺体积逐渐缩小，腺上皮细胞逐渐萎缩，核染色加深，腺泡萎缩甚至闭塞，间质增生，加用羟氯喹处理后这一变化更加明显。电镜下观察去势后自噬小体的数量明显增加。免疫组化检测去势后自噬活性蛋白P62表达减弱，用羟氯喹处理后表达增强；凋亡促进蛋白Caspase-3表达增强，用羟氯喹处理后表达进一步增强。结论去势后大鼠前列腺细胞自噬增加，自噬抑制剂羟氯喹可通过阻断细胞自噬增加大鼠细胞凋亡，自噬可能具有抗凋亡作用。Objectives To discuss the influence of blocking autophagy to apoptosis in rat prostate after castration. Methods Seventy - two healthy male SD rats were randomly divided into 3 groups ： the sham operation group, castration group and hydroxychloroquine group. Each group contains 24 rats. After 24 hours, 72hours, 7days, 10days, 14days and 21 days of the operation, we took out four rats randomly from each group and removed the protastes. Then we observed the morphological changes of the prostates through optical microscope and the changes of cytolysosomes and apoptotic bodies in the prostates by electronic speculum. Analyzed the expression of the proteins of P62 and Caspase -3 by immunohistochemistry. Results The prostate tissues shranked remarkably in the castrated rats. Cytolysosomes were observed through electronic speculum. The expressoin of Caspase - 3 increased significantly （ P 〈 0.05 ） in those that had received castration, whereas P62 decreased significantly （ P 〈 0.05 ）. Conclusions Autophagy protect protaste from apoptosis, and apoptosis would increase after dealing with choroquine, which would provide new therapies for drug - resistant prostate cancer.

关 键 词：前列腺 细胞凋亡 自噬 大鼠 

分 类 号：R697.3[医药卫生—泌尿科学]