拟南芥中磷脂酶Dδ参与机械伤害诱导的磷脂酸生成（英文）  

Phospholipase Dδ is Involved in Wounding-Induced Phosphatidic Acid Formation in Arabidopsis

作　　者：李爱花[1,2] 凌立贞[1] 李唯奇[1] 

机构地区：[1]中国科学院昆明植物研究所中国西南野生生物种质资源库,昆明650201 [2]中国科学院大学,北京100049

出　　处：《植物分类与资源学报》2015年第4期428-438,共11页Plant Diversity

基　　金：The National Science Foundation of China(NSFC31300261)

摘　　要：磷脂酶水解磷脂产生磷脂酸(phosphatidic acid,PA),Dα1和δ是磷脂酶D家族中表达丰度最高的两个成员,已知磷脂酶Dα1参与了机械伤害诱导的磷脂酸信号,但是磷脂酶Dδ是否以及如何参与PA信号尚且未知。本研究利用脂类组学分析方法,比较了拟南芥野生型(WS)和磷脂酶Dδ基因T-DNA插入突变体(PLDδ-KO),在机械伤害后的较长时间段(6 h)的膜脂分子变化。结果发现,机械伤害后,拟南芥两种基因型的大部分膜脂均发生下降,且机械伤害后30 min,PA含量即快速并急剧升高;随着时间的延长,其水平持续升高,直至达到峰值后下降至6 h达到最低值。WS和PLDδ-KO达到PA最高值的时间不同,分别为1 h和3 h;在伤害处理后30 min至3 h期间,PLDδ-KO中的PA水平低于WS,两个基因型中的PA含量最大差值为20%,发生在伤害后1 h。这证明缺失PLDδ基因在一定程度抑制了机械伤害诱导的PA生产,表明PLDδ参与拟南芥响应机械伤害的PA生成,但是其响应较PLDα1作用慢且轻。这是PLDδ响应拟南芥中机械伤害的首次报道。Phosphalipase D （PLD） hydrolyzes phospholipids into phosphatidic acid （PA）. PLDcd and 5 are the two most abundant members of the 12-member PLD family in Arabgdopsis. PLDαl has been demonstrated having role in the wounding-induced PA signalling. However, whether and how PLD5 is involved in wounding-induced PA for- mation remained unclear. In the present study, the membrane lipids response to wounding was profiled in Wassil- ewskija （WS） and PLD8 knockout mutant （PLDS-KO） of Arabidopsis. The levels of most lipids, including mo- nogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylcholine and phosphatidylglycerol had decreased rapidly within 30 min after wounding in the two Arabidopsis genotypes. In contrast, the level of PA increased sharply and significantly 30 min after wounding. It continued to increase until peaking at 1 h post-wounding in WS and 3 h post-wounding in PLDδ-KO, and then decreased. The PA levels were similar in the two genotypes in untreated leav- es and in leaves 6 h after wounding. However, these levels were lower in PLDδ-KO than in WS from 30 min to 3 h post-wounding. The significant difference of PA level between the two genotypes occurred 30 min after wounding, when it was about 20% lower in PLDδ-KO than in WS. These results show that PLD5 is involved in wounding-in- duced PA formation in Arabidopsis, but its absence induces PA response later and with less intensity than PLDα1.

关 键 词：拟南芥 机械伤害 膜脂 磷脂酶Dδ 磷脂酸 

分 类 号：Q946[生物学—植物学]