拟南芥Rboh基因家族成员的分子和功能学比较（英文）  

Molecular and Functional Comparisons of Reactive Burst Oxygen Species Gene Family in Arabidopsis

作　　者：孙旭东[1,2] 胡向阳[1,2] 杨永平[1,2] 

机构地区：[1]中国科学院昆明植物研究所东亚植物多样性与生物地理学重点实验室,昆明650201 [2]中国科学院昆明植物研究所西南野生生物种质资源库,昆明650201

出　　处：《植物分类与资源学报》2015年第4期463-471,共9页Plant Diversity

基　　金：The National Natural Science Foundation of China(NSFC 31400244)

摘　　要：活性氧(reactive oxygen species,ROS)在植物的信号转导中起着重要的作用。它们参与了植物的生长发育,生物及非生物胁迫和细胞死亡等过程。最近的研究发现呼吸爆发氧化酶(Respiratory burst oxidase homologues,Rboh)是植物ROS的主要生产者。拟南芥Rboh基因家族由10个成员组成,他们编码的蛋白包含6个跨膜结构域、以及C末端的FAD与NADPH亲水结构域和N末端的2个Ca2+结合EF手性结构。本文通过聚类分析发现拟南芥Rboh基因家族成员的三个分枝具有高度的同源性,这说明拟南芥Rboh家族成员间可能存在功能冗余。利用RT-PCR分析了各基因成员的时空表达特性,并对整个家族成员的缺失突变体进行表型分析发现,除了rboh C外,都没有明显的表型变化,这说明拟南芥Rboh C可能在植物发育过程中具有特殊的功能。Reactive oxygen species （ROS） play a key role in cell signal transduction. They are involved in the regu- lation of growth, development, responses to abiotie or biotic stimuli and cell death. Recent studies identified respira- tory burst oxidase homologues （RBOHs） as the key producers of ROS. The Arabidopsis genome contains 10 Rboh genes, that encode proteins with six transmembrane domains supporting two haem groups, FAD and NADPH hydro- philic domains in the C-terminal region and two calcium-binding domains （EF-hand） in the N-terminal region. In the present study, we investigated patterns of transcription systematically in Arabidopsis plants. Comparisons among amino acid sequences of ten Rboh genes revealed high degrees of identity among entire amino acid sequences of three groups, suggesting that some members of Rboh family might have redundant functions. With the except of rbohC, none of the loss-of-function mutants was found to display distinct phenotypes, suggesting that RbohC might have a specific function in plant development.

关 键 词：NADPH氧化酶 呼吸爆发氧化酶基因 聚类分析 T-PCR 功能冗余 

分 类 号：Q943.2[生物学—植物学]