丙泊酚麻醉对老年大鼠记忆功能影响的行为学研究  

Effects of propofol anesthesia on cognitive function of aged rats

作　　者：仝晶晶[1] 杨一晨[1] 司家瑞[2] 汤晓红[3] 徐新宇[1] 

机构地区：[1]天津医科大学生物医学工程学院医学神经生物学实验室,300070 [2]天津医科大学基础医学院,300070 [3]天津医科大学总医院麻醉科、天津市麻醉学研究所,300073

出　　处：《国际生物医学工程杂志》2015年第3期129-132,共4页International Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目（61301252）;中国博士后科学基金资助项目（2014M561193）

摘　　要：目的研究丙泊酚麻醉对老年大鼠认知功能的影响。方法选取96只雄性SD老年大鼠（16月龄）为研究对象，在丙泊酚麻醉后第7、30、90天，应用恐惧记忆实验测试老年大鼠长期记忆功能（每个时间节点各12点，共36只）；在麻醉后5d内应用自主空间探索实验测试老年大鼠工作记忆功能（每个时间节点各12点，共60只）。结果在麻醉后7、30、90d，丙泊酚组与对照组老年大鼠长期记忆功能的差异均无统计学意义（P〉0．05）。麻醉后2d内，丙泊酚组老年大鼠工作记忆功能受损（P〈0．05）；在第3、4、5天，2组老年大鼠空间工作记忆差异均无统计学意义（P〉0．05）。结论实验结果表明临床剂量丙泊酚麻醉会导致老年大鼠48h内的工作记忆功能损伤，但不会损伤其长期记忆功能。Objective To investigate the effects of propofol anesthesia on cognitive function of aged rats. Methods Ninety-six male aged SD rats （16 months） were collected and given propofol anesthesia via tail vein catheter. At 7, 30, and 90 d after anesthesia, fear conditioning experiment Was performed to test long-term memory of the aged rats （12 rats at each time point, total 36 rats）. At 1, 2, 3, 4, and 5 d after anesthesia, spontaneous alternation in Y-maze experiment was performed to test spatial working memory of the aged rats （12 rats at each time point, total 60 rats）. Results There were no statistical differences in long-term memory at 7, 30, and 90 d after anesthesia between the propofol group and control group （P〉0.05）. While spatial working memory of aged rats in propofol group was impaired at 1 and 2 d after anesthesia （P〈0.05）, working memory of aged rats in propofol group was normal at 3, 4, and 5 d after anesthesia and there were no statistical differences between the experiment and control group （P〉0.05）. Conclusions These results indicate that clinical dose propofol anesthesia will not induce long-term memory impairment of aged rats, although it impairs spatial working memory of aged rats within 48 h after anesthesia.

关 键 词：丙泊酚 老年 工作记忆 长期记忆 

分 类 号：R318[医药卫生—生物医学工程] R971.2[医药卫生—基础医学]