不同浓度激素配比对高羊茅再生体系建立的影响  

Effects of Different Concentrations of Hormones on the Regeneration System of Tall Fescue

作　　者：郭伶娜 刘建文[2] 麻冬梅[2] 许兴[2] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]宁夏大学农学院,宁夏银川750021

出　　处：《草地学报》2015年第3期517-525,共9页Acta Agrestia Sinica

基　　金：国家"973"项目(2012CB114204);自治区牧草育种专项(NYYZ-2014-Z12)资助

摘　　要：以‘爱瑞3号'为试材,以无菌种子为外植体,对成熟种子愈伤组织诱导、胚性愈伤组织继代和分化的影响因素进行了研究,构建了高频的高羊茅胚性愈伤组织植株再生体系。结果表明:最佳愈伤诱导培养基配方为MS+5.5 mg·L^(-1)2,4-D+500 mg·L^(-1)CH;最佳分化培养基配方为MS+2.0 mg·L^(-1)6-BA+1.0 mg·L^(-1)KT;最佳生根培养基配方为1/2MS+0.3 mg·L^(-1)BA,其蔗糖含量为20 g·L^(-1)。The impacts of manifold factors on callus induction from mature seeds, subculture production and embryogenic calli differentiation were studied here. Plant regeneration system of tall rescue （Festuca arundinacea Schreb. ） embryogenic calli was established using the sterile seeds of Arid 3 variety as explants. The results showed that the best callus induction medium was MS＋5. 5 mg ·L^-1 2,4-D＋500 mg ·L^-1 CH; and MS＋2.0 mg ·L^-1 6-BA＋1. 0 mg ·L^-1 KT medium performed the best function in the differentiation of tall fescue embryogenic calli. The best rooting medium was 1/2MS＋0.3 mg ·L^-1 IBA, and the content of sucrose was 20 g·L^-1.

关 键 词：草坪草 组织培养 再生体系 

分 类 号：S543.9[农业科学—作物学]