超高效液相色谱-蒸发光散射检测器法测定参松养心胶囊中人参皂苷Rg_1和Re含量  

UPLC-ELSD Method for Determination of Ginsenoside Rg_1 and Re in Shensong Yangxin Capsule

作　　者：陈小娟 李志红 刘静 刘贺 

机构地区：[1]北京以岭药业有限公司,北京102600

出　　处：《中国药业》2015年第14期84-86,共3页China Pharmaceuticals

摘　　要：目的建立测定参松养心胶囊中人参皂苷Rg1和Re含量的超高效液相色谱-蒸发光散射检测器（UPLC-ELSD）法。方法采用Waters ACQUITY UPLC BEH C18柱（100 mm×2.1 mm,1.7μm）为色谱柱,以乙腈-水为流动相（梯度洗脱）,流速为0.45 m L/min,柱温为35℃,进样量为2μL,ACQUITY ELSD检测器的漂移管温度为50℃,气体流量为30.0 psi。结果参松养心胶囊中人参皂苷Rg1和Re进样量分别在0.091 08~1.135 8μg和0.083 06~1.038 25μg范围内与峰面积线性关系良好,平均加样回收率分别为99.01%和99.25%,RSD分别为0.50%和0.36%（n=6）。结论与高效液相色谱（HPLC）法相比,UPLC法在不影响分离效果的情况下大大提高了参松养心胶囊中人参皂苷Rg1和Re的分离速度,同时减少了溶剂消耗。UPLC法可作为替代传统HPLC法的一种更方便、快捷、可靠的方法 。Objective To establish a ultra performance liquid chromatography- evaporative light scattering detector（ UPLC- ELSD） method for determining the ginsenoside Rg1 and Re contents in Shensong Yangxin Capsule. Methods The Waters ACQUITY UPLC BEH C18column（ 100 mm × 2. 1 mm,1. 7 μm） was adopted. The mobile phase consisted of acetonitrile- water with gradient elution. The flow rate was 0. 45 m L / min,the column temperature was 35 ℃ and the injection volume was 2 μL. As to the ELSD,the temperature of drift tube was 50 ℃ and the flow rate of carrier gas was 30. 0 psi. Results The sample seizes of ginsenoside Rg1 and Re in Shensong Yangxin Capsule within the range of 0. 091 08- 1. 135 8 μg and 0. 083 06- 1. 038 25 μg had good linear relation with the peak area,respectively. And the average recovery rates were 99. 01% and 99. 25%,RSD 0. 50% and 0. 37%（ n = 6）,respectively.Conclusion Compared with HPLC,the UPLC method may greatly improve the analytical speed of ginsenoside Rg1 and Re in Shensong Yangxin Capsule without affecting the separation effect,at the same time reduce the solvent consumption. As an alternative of conventional HPLC,UPLC is more convenient,rapid and reliable.

关 键 词：超高效液相色谱-蒸发光散射检测器法 参松养心胶囊 人参皂苷RG1 人参皂苷RE 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]