巴西橡胶树锌指蛋白基因的克隆及功能鉴定  

Cloning and Characterization of a Zinc-finger Gene of Hevea brasiliensis

作　　者：肖华[1] 林云蒸 姚笛[1] 欧阳沫 黄惜[1] 

机构地区：[1]海南大学农学院/海南省热带生物资源可持续利用重点实验室,海南海口570228

出　　处：《热带生物学报》2015年第2期113-118,共6页Journal of Tropical Biology

基　　金：海南省科技项目(ZDZX2013023);国家自然科学基金(31370608;31260170)资助

摘　　要：锌指蛋白是一类能与DNA/RNA结合并调控基因表达的蛋白.前期笔者通过酵母双杂技术筛选到1个可能与JAZ蛋白互作的巴西橡胶树锌指蛋白（命名为Hbzinc-finger）,该基因的cDNA全长序列有2 262bp,编码753个氨基酸.该蛋白碳末端有1个PB1结构域,在336 ～381处氨基酸序列编码1个ZnF_ZZ锌离子结合结构域.红色荧光蛋白亚细胞定位结果表明,Hbzinc-finger定位于细胞核,酵母自转录激活显示该蛋白有自转录活性.该基因的表达受创伤影响,表明橡胶锌指蛋白的表达可能受茉莉酸信号调控.Zinc-finger is a protein that binds DNA/RNA and regulates gene expression. In previous work, we i- dentified a Zinc-finger protein （ termed as HbZinc-finger） that interacted with HbJAZ1 in Y2H against rubber la- tex cDNA yeast library. The full-length cDNA of HbZinc-finger gene is 2 262 bp in length and encodes 753 aa. C-terminal of HbZinc-finger contains a PB1 domain. A ZnF_ZZ domain exists between 336 aa and 381 aa. Sub- cellular fluorescent localization result showed that the HbZinc-finger was located in nucleus. Additionally, HbZ- inc-finger showed strong self transcriptional activation in yeast. Furthermore, the expression of HbZinc-finger was affected by continuously wounding in rubber latex, suggesting that HbZinc-finger is possibly regulated by the JA signaling pathway

关 键 词：HbZinc-finger 克隆 亚细胞定位 自转录激活 表达 

分 类 号：Q816[生物学—生物工程]