珠江流域黄颡鱼群体的形态度量学与遗传多样性分析  

Morphology and Genetic Diversity Analysis of Pelteobagrus fulvidraco Populations in Zhujiang River

作　　者：谢堂晖 赵会宏[1] 杨慧荣[1] 刘丽[1] 韩兴鹏[1] 杨宪宽 陈奕彬[1] 刘丽杰[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642

出　　处：《中山大学学报（自然科学版）》2015年第4期112-120,共9页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：公益性行业(农业)科研专项经费资助项目(201203066);广东省教育部产学研结合资助项目(2012B091100484);广东省教育部科技部产学研结合资助项目(2012B090600052);广东省科技计划资助项目(2012B040400004);广东省海洋渔业科技推广专项资助项目(A201201E04);广东省协同创新与平台环境建设资助项目

摘　　要：为了解珠江流域黄颡鱼不同群体的遗传分化,以黄颡鱼为材料,通过形态度量学和分子生物学的方法对其进行研究。形态度量学的主成分分析(PCA)的散点图显示都柳江群体与漓江群体聚集于一处,东江群体与都柳江、漓江群体分开,表明东江群体与其它两个群体形态上有分化,漓江群体与都柳江群体形态间分化较小。D-loop序列分析最终检测到176个变异位点,单倍型36个,3个群体的平均碱基组成差异很小,且A+T含量(59.6%)高于G+C含量(40.4%),3个群体平均Nm=3.234,表明群体间存在较大基因流,单倍型NJ树显示漓江群体与都柳江群体聚为一支,东江群体聚为一支。3个群体21个微卫星标记位点的分析结果显示:3个群体有15个位点属于高度多态位点(PIC>0.5),3个群体基于Nei's遗传距离构建的UAPGM聚类结果与mt DNA D-loop聚类结果一致,与地理分布呈一定相关性。综合形态度量学与分子生物学标记结果可以看出,黄颡鱼3个群体存在一定形态差异,3个黄颡鱼野生群体遗传多样性水平较高。Genetic differences of Pelteobagrus fulvidraco populations from Zhujiang River were compared by morphological and molecular biological methods. The scatter diagram of principal component analysis revealed that the populations from Duliujiang River and Lijiang River pooled into one group,while those from Dongjiang River separated from Duliujiang River and Lijiang River. It indicated that Dongjiang River population had a certain morphological differentiations compared to other two populations,while there were few morphological differentiation between Duliujiang River and Lijiang River. The mt DNA D-loop sequence analysis of three populations showed that 176 variable sites and 36 haploid types were detected,there were similar base compositions among three populations,the content of A ＋ T（59. 6%） was higher than the content of G ＋ C（40. 4%）,the average gene flow was 3. 234,there was a large gene exchange between three populations. The NJ tree of haploid type shows that the populations Lijiang River and Duliujiang River formed the first cluster which together with the populations from Dongjiang River formed the second cluster. The analyzed result of 21 microsatellite loci in three populations showed that15 loci were high polymorphic（ PIC 0. 5）,the clustering result of three populations was in accordance with the result of mt DNA D-loop marker,all clustering results had a certain correlation with their geographical distribution. From the result of morphological and molecular analysis, a conclusion can be drawn that the morphology in three populations had certain difference,there was rich genetic diversity among populations.

关 键 词：黄颡鱼 珠江流域 形态度量学 MTDNA D-LOOP 微卫星 

分 类 号：S91[农业科学—水产科学]