气相色谱法分析大麻药多糖成分中单糖组成  

Analysis of Monosaccharides Compositions of Polysaccharide in Dolichos falcatas Klein. by Gas Chromatography

作　　者：邓旭坤[1] 吴非[1] 陈旅翼[1] 林亲雄[1] 罗凯[1] 郑慧山 

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2015年第2期48-51,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家科技部"十二五"科技支撑计划资助项目(2012BAI27B06);国家自然科学基金资助项目(81073147)

摘　　要：为研究大麻药多糖成分中单糖的组成,用3 mol·L-1三氟乙酸将大麻药多糖水解成单糖,再与盐酸羟胺进行衍生化反应,用气相色谱法分析了其单糖组成,结果表明:鼠李糖、阿拉伯糖、果糖、葡萄糖、阿洛糖和半乳糖为大麻药多糖成分中的单糖组成,其质量分数依次为15.86%,20.37%,6.62%,14.24%,8.38%和14.15%.此方法简便、快速,重现性好,可用于大麻药多糖成分中单糖的组成分析.To analyze the monosaccharide composition of polysaccharide in Radix Dolichosae,the polysaccharide was hydrolyzed into monosaccharides with 3 mol ·L^-1 trifluoroacetic acid, and then derivatized with hydroxylamine hydrochloride. The monosaccharide composition and content were analyzed by gas chromatography. The results indicated that the monosaccharides in Radix Dolichosae polysaccharide were mainly composed of rhamnose,arabinose,fructose,glucose,allose and galactose with a mass fraction of 15. 86%,20. 37%,6. 62%,14. 24%,8. 38% and 14. 15%,respectively. This method was simple,rapid and reproducible,which could be effectively applied to the analysis of monosaccharide composition of Radix Dolichosae polysaccharide.

关 键 词：气相色谱法 大麻药 多糖 单糖 

分 类 号：R917[医药卫生—药物分析学]