美国红叶紫薇的组织培养与快速繁殖  

Tissue Culture and Rapid Micropropagation of Lagerstroemia indica ‘Pink Velour'

作　　者：陈怡佳[1] 崔媛媛[1] 张晓明[1] 邓小梅[1] 

机构地区：[1]华南农业大学林学与风景园林学院广东省森林植物种质创新与应用重点实验室,广州510642

出　　处：《植物生理学报》2015年第6期882-886,共5页Plant Physiology Journal

基　　金：国家林业局林业公益性行业科研专项(201404116)

摘　　要：以美国红叶紫薇优株当年生半木质化枝条为外植体,采用丛芽发生途径,建立其组培快繁技术体系。结果表明,最适腋芽诱导培养基为MS＋6-BA 1.0 mg·L-1＋IBA 0.05 mg·L-1,诱导率高达88%;最佳增殖培养基为ZW（改良的WPM）＋6-BA 0.5mg·L-1＋IBA 0.05 mg·L-1,增殖系数可达5.21,芽高4.6 cm;生根最适培养基为ZW＋IBA 0.5 mg·L-1,生根率100%,平均每株苗生根4~5条。炼苗后,移栽于泥炭土和珍珠岩3：1（V/V）的混合基质中,成活率高达95%以上。Tissue cultural techniques for Lagerstroemia indica ‘Pink Velour＇ were studied through clustered bud cultivation with 1-year-old semi-lignified shoots as explants. The results showed that the suitable induction medium was MS medium containing 1.0 mg·L-1 6-BA and 0.05 mg·L-1 IBA, the induction rate of which reached 88%. The suitable proliferation medium with the robust and orderly buds was ZW（modified WPM） medium containing 0.5 mg·L-1 6-BA and 0.05 mg·L-1 IBA, the multiplication coefficient of which was 5.21 roughly. The suitable rooting medium was ZW medium containing 0.5 mg·L-1 IBA, the rooting rate of which reached 100%. The transplanting matrix were peat and pearlite（3：1, V/V）, and the transplanting survival rate of which was up to 95%.

关 键 词：美国红叶紫薇 组织培养 基本培养基 快速繁殖 

分 类 号：S685.99[农业科学—观赏园艺]