高温、强光下黄瓜叶片PSII供体侧和受体侧的伤害程度与快速荧光参数W_k变化的关系  

The Relationship between the Changes in W_k and Different Damage Degree of PSII Donor Side and Acceptor Side under High Temperature with High Light in Cucumber

作　　者：金立桥 车兴凯 张子山[1] 高辉远[1] 

机构地区：[1]山东农业大学生命科学学院/作物生物学国家重点实验室,山东泰安271018

出　　处：《植物生理学报》2015年第6期969-976,共8页Plant Physiology Journal

基　　金：高等学校博士学科点专项科研基金项目(20113702110008);国家自然科学基金(31370276)

摘　　要：快速荧光OJIP曲线中的Wk参数被广泛作为放氧复合体(OEC)即PSII供体侧伤害的重要指标。然而Wk这一参数的变化不仅受PSII供体侧伤害的影响,而且还会受到PSII受体侧伤害的影响。很多研究者在使用Wk这个参数时忽略了这个问题。为了阐明PSII供体侧和受体侧伤害程度对Wk影响的规律,我们通过不同温度和强光处理,获得不同PSII供体侧和受体侧伤害的黄瓜叶片,进而分析Wk变化与其供体侧和受体侧伤害程度之间的关系。研究结果表明:用高温黑暗处理黄瓜叶片后,其PSII供体侧受到严重的伤害,此时,Wk随着供体侧伤害程度的增加而显著增加,在这种情况下,Wk的增加直接反映了OEC的伤害程度;但是当用强光和高温共同处理黄瓜叶片后,造成PSII供体侧和受体侧同时伤害时,如果供体侧的伤害程度大于受体侧的伤害程度时,Wk仍然升高,然而,当受体侧的伤害程度大于供体测定伤害程度时,Wk不再显著升高。因此在植物叶片的供体侧和受体共同受到伤害时,要慎重使用Wk这一参数来判断OEC的伤害程度。本文对Wk在不同供体侧和受体侧伤害条件下的变化规律进行了详细的研究,并对如何正确使用Wk来反映逆境对植物叶片OEC的伤害程度进行了探讨,该研究结果可以对正确利用叶绿素快速荧光OJIP分析提供指导。Wk, a parameter of chlorophyll（Chl） a fl uorescence OJIP curve, is an important indicator of OEC damages. However, Wk is not only effected by the damage of PSII donor side but also by the damage of PSII acceptor side. Many researchers ignored this fact. To explore the relationship between the changes in Wk and the damage of PSII donor side and acceptor side, we treated leaves of cucumber with different duration of high temperature with high light. We analyzed the relationship between Wk and the damage degree of PSII donor side and acceptor side in leaves of cucumber. Our results demonstrated that when the leaves of cucumber were treated with high temperature in the dark, severe damage of the donor side in leaves occurred. In this situation, Wk increased with the damage degree of the donor side, which directly refl ected the damage degree of the OEC. However, when the leaves of cucumber were treated with high light with high temperature, the donor side and the acceptor side were both injured. In this situation, when the damage degree of the donor side was more sever than that of the acceptor side, Wk would still increase; but when the damage degree of the acceptor side was more sever than that of the donor side, Wk would not increase any more. So when the donor side and the acceptor side were both damaged, Wk should be used carefully to refl ect the damage degree of OEC. The relationship between changes in Wk and the damage degree of donor side and acceptor side was studied in detail, the conclusion of the study may instruct users of OJIP to use Wk correctly to refl ect the damage degree of OEC under stresses.

关 键 词：快速叶绿素荧光 PSII供体侧 PSII受体侧 放氧复合体(OEC) Wk 

分 类 号：S642.2[农业科学—蔬菜学]