朊病毒感染与内源性嘌呤受体P2X7相互关系的研究  

Association between prion infection and endogenous purinoceptor P2X7 in experimental rodents and SMB-S15 cells

作　　者：王晶[1] 张宝云[1] 肖康[1] 陈操[1] 陈利娜[1] 周伟[1] 董小平[1] 石琦[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京102206

出　　处：《中华实验和临床病毒学杂志》2015年第3期216-218,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金（81301429）;2014年度留学人员科技活动项目择优资助（人专司函[2013]167号）

摘　　要：目的 明确朊病毒感染对P2X7受体的影响,并评价P2X7受体活化对朊病毒复制的作用.方法 Western Blot检测朊病毒毒株263K感染的仓鼠、ME7和139A感染的C57 BL/6小鼠和稳定感染羊瘙痒因子Chandler株的小鼠神经细胞系SMB-S15中P2X7受体的蛋白表达,并检测P2X7激活剂ATP对SMB-S15细胞中朊病毒PrPSc含量的影响.结果 朊病毒感染的动物及细胞模型中,P2X7受体表达降低（P＜0.05）,ATP孵育4d可降低SMB-S15细胞中PrPSc的含量.结论 P2X7信号通路活化对朊病毒感染细胞具有一定的保护作用.嘌呤信号通路有望为抗朊病毒治疗提供新的靶点。Objective To assess the impact of prion infection on purinoceptor P2X7,and evaluate the role of P2X7 activation during prion replication.Methods The expression of P2X7 in scrapie strain 263K-infected hamsters,strains 139A and ME7-infected mice and prion infected cell line SMB-S15 analyzed by Western Blot and the influence of P2X7 activation on the level of PrPSc in SMB-S15 cells after 4-day-incubation with ATP was observed.Results P2X7 expression was down-regulated by prion infection and activation of P2X7 by ATP decreased the amount of PrPSc in SMB-S15 cells.Conclusions P2X7 receptor and its downstream signaling pathways could play important protective roles during prion infection,which highlights its therapeutic potential in prion diseases.

关 键 词：受体 嘌呤能 朊病毒 腺苷三磷酸 

分 类 号：R373[医药卫生—病原生物学]