发热伴血小板减少综合征病毒感染Balb/C小鼠和金黄地鼠的免疫病理反应  

Infection of the Severe Fever with Thrombocytopenia Syndrome Virus in Balb/C Mice and Hamsters

作　　者：金聪[1] 韩颖[1,2] 李川[1] 顾雯[1] 蒋虹[3] 陈霆[3] 朱华[3] 魏强[3] 仇佩虹[2] 梁米芳[1] 李德新[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206 [2]温州医学院药学院,温州325003 [3]中国医学科学院实验动物研究所,北京100021

出　　处：《病毒学报》2015年第4期379-387,共9页Chinese Journal of Virology

基　　金：艾滋病和病毒性肝炎等重大传染病防治项目:重大传染病应急处置检测技术平台(2013ZX10004-101);自然科学基金(通过动物感染模型研究新型布尼亚病毒诱发血小板减少的病理机制81101301)

摘　　要：发热伴血小板减少综合征病毒(Severe fever with thrombocytopenia syndrome irus,SFTSV)是新发传染病发热伴血小板减少综合征的致病病原,为布尼亚病毒科白蛉病毒属的一种新型病毒。为了分析SFTSV感染Balb/C小鼠和金黄地鼠引起的免疫病理反应,本研究采用两个攻毒剂量组分为高剂量组(105 TCID50),低剂量组(103TCID50),以及溶剂对照组,并采用经静脉、肌肉、脑、和腹腔4种途径分别进行攻毒。在攻毒后不同时间点取血样,应用全自动血细胞计数仪检测血细胞亚群,Real-time PCR实验检测血液中病毒RNA拷贝数,悬浮芯片实验检测血浆中IgG和IgM抗体水平。在攻毒后第14d处死动物,收集心、肝、脾、肺、肾、小肠、肌肉和脑8种组织器官进行HE染色,观察组织病理改变。研究结果显示,SFTSV可感染Balb/C小鼠和金黄地鼠,在攻毒后第7d检测到病毒特异性IgM和IgG,其中IgM在第7d达到峰值后下降,IgG在攻毒后第14d达到峰值。组织病理学分析显示病毒感染Balb/C小鼠和金黄地鼠的肝组织和肾组织出现明显病变。本研究揭示SFTSV可以感染不同品系的啮齿类动物,引起相似的免疫抗体反应和组织病理变化。The severe fever with thrombocytopenia syndrome virus（SFTSV）is the causative pathogen of an emerging infectious disease severe fever with thrombocytopenia syndrome and a new member in the genus Phlebovirus of family Bunyaviridae.Immune responses and pathological lesions in SFTSV-infected Balb/C mice and hamsters were evaluated by inoculation of SFTSV at 105 TCID50 or 103 TCID50 per animal through four different routes of infection,including intravenous,intramuscular,intraperitoneal,and intracerebral injections.The vehicle control groups were also included.At different time points after the inoculation blood and plasma samples were collected.Blood cell counts,blood viral RNA copies,and plasma antibodies were detected by automatic blood cell counters,real-time PCR,and luminex assays,respectively.At two weeks post inoculation,the animals were sacrificed.Tissues including heart,liver,spleen,lung,kidney,intestine,muscle,and brain,were collected for pathological analyses.Results showed that the SFTSV could infect Balb/C mice and hamsters with SFTSV-specific immunoglobulin（Ig）M and IgG antibodies detected in plasma samples on day 7post inoculation.The SFTSV-specific IgM levels peaked on day 7post inoculation and then decreased,whereas the SFTSV-specific IgG levels started to increase on day 7and then peaked on day 14 post inoculation.Pathological analyses indicated significant pathological lesions in liver and kidney tissues.In conclusion,SFTSV could can infect different strains of rodent animals and cause similar immunological and pathological responses.

关 键 词：发热伴血小板减少综合征病毒 BALB/C小鼠 金黄地鼠 免疫病理反应 动物感染模型 

分 类 号：R373.32[医药卫生—病原生物学]