辽宁省1997～2014年H1a基因亚型麻疹野病毒流行株血凝素(H)基因特征分析  

Genetic Characterization of Hemagglutinin on Measles Virus Epidemic Strain Genotype H1a in Liaoning Province(China) from 1997 to 2014

作　　者：王艳[1] 马艳[1] 徐晓婷[1] 郝爽[1] 韩悦[1] 姚文清[1] 赵卓[1] 

机构地区：[1]辽宁省疾病预防控制中心,沈阳110005

出　　处：《病毒学报》2015年第4期410-419,共10页Chinese Journal of Virology

基　　金：国家科技重大专项"传染病监测技术平台"项目(项目编号:2012ZX10004209)

摘　　要：了解辽宁省1997~2014年流行麻疹野病毒血凝素（Hemagglutinin,H）基因特征,为控制和消除麻疹提供依据。本研究选用1997~2014年分离的63株H1a基因亚型麻疹病毒（Measles,MEV）,应用逆转录-聚合酶链反应（RT-PCR）分别扩增麻疹病毒（MEV）血凝素（H）基因全长和N基因的450个核苷酸片段,对扩增产物进行核苷酸序列测定和分析。将辽宁省63株MEV序列与GenBank中收录的1993~1994年麻疹病毒H基因型代表株、中国疫苗株沪191（S-191）和长春47（C-47）基于H全长构建基因亲缘性关系树,进行核苷酸和氨基酸变异分析。结果显示辽宁省1997~2014年63株MEV均属于H1基因型的H1a亚型,并分为两个簇（cluster 1和2）。H基因的进化速度低于N基因的进化速度。所有63株MeV在H基因第240位均由丝氨酸（Ser S）突变为天冬酰胺酸（Asn N）,在243位由精氨酸（Arg R）突变成甘氨酸（Gly G）,第481位由酪氨酸（Tyr Y）突变为天冬酰胺酸（Asn N）,另外在397位上cluster 2毒株均由脯氨酸（Pro P）突变成亮氨酸（Leu L）,其他具有生物学和免疫学活性的位点未发生改变。To wished to characterize the hemagglutinin（H）gene of the measles virus epidemic strain H1 ain Liaoning Province（China）from 1997–2014to provide a basis for the control and elimination of measles.All 63 measles virus strains were the H1 agenotype.Fragments of the H gene（1854nucleotides）and nucleoprotein（N）gene（450nucleotides）were amplified by reverse transcription-polymerase chain reaction（RT-PCR）and the PCR products sequenced and analyzed.Phylogenetic trees were constructed with reference strains of the genotype-H measles virus downloaded from GenBank,including Chinese measles virus strains isolated in 1993–1994and the vaccine reference strains S-191 and C-47.Sixty-three strains of the measles virus in 1997–2014belonged to genotype H1 a.The mean evolutionary rate for gene N-450 was higher than that for the H gene.All 63 strains of the measles virus were mutated from：serine（Ser S）to asparagine（Asn N）in the 240 th amino acid;arginine（Arg R）to glycine（Gly G）in the 243th;and tyrosine（Tyr Y）to Asn N in the 481 th amino acid.All measles virus strains in cluster 2were mutated from proline（Pro P）to leucine（Leu L）in the 397 th amino acid.The other neutralization sites showed no apparent difference when comparing the nucleotides/amino acids of the H gene of S191 vaccine strains.

关 键 词：麻疹病毒 HA基因 分析 

分 类 号：R373.11[医药卫生—病原生物学]