衣原体噬菌体衣壳蛋白3的结构、免疫原性及临床价值研究  

Structure,Immunogenicity and Clinical Value of Chlamydiaphage Capsid Protein 3

作　　者：姚卫锋[1] 李宜儒[2] 袁静[1] 王磊[1] 李群燕[1] 宋蒙蒙[1] 卢桂玲[1] 张理涛[1] 

机构地区：[1]天津市中医药研究院附属医院,天津300120 [2]安徽医科大学,合肥230032

出　　处：《病毒学报》2015年第4期420-424,共5页Chinese Journal of Virology

基　　金：天津市中医药管理局基础研究资助项目(编号:13017)

摘　　要：分析衣原体微病毒Vp3蛋白在分子重组、分子进化及病毒野生株筛查的作用并挖掘生物信息,揭示其临床研究价值。以ProteinBlast的Multriple alignment程序比对各株衣原体微病毒衣壳蛋白Vp3序列;以Distance tree(ProteinBlast)程序完成种系发生树。以比对获得的高保守区的氨基酸序列为目标,应用Karplus-schulz法进行柔性区域分析;通过Gamier-robson法和Chou-fasman法分析该序列的二级结构;使用Emini法完成表位的表面可及性分析,并用Kyte-Doolittle法和Hopp-woods法完成蛋白亲水性研究;应用Jameson-Wolf法完成表位的抗原指数分析。6株衣原体微病毒Vp3蛋白序列高度保守,差异主要在Chp1与其他5株微病毒的Vp3蛋白之间。各株微病毒的Vp3蛋白均有以α螺旋为主的结构,蛋白序列高保守区存在多个细胞表位。Vp3蛋白结构性质保守,也是衣原体噬菌体衣壳的重要组分。其蛋白分子结构复杂,高保守区有较强的免疫原性,在分子重组、沙眼衣原体微病毒野生株筛查研究中有实际研究价值。We wished to assess the role of chlamydia micro virus capsid protein Vp3 in recombinant molecules,chart its molecular evolution,screen the wild-type strain,and reveal its value in clinical research.Using aprotein BLAST multiple-alignment program,we compared various strains of Chlamydia micro virus capsid protein Vp3 sequences.Using a＂distance tree＂of those results,we created a phylogenetic tree.We applied the Karplus-Schulz method of flexible-region analyses for highly conserved alignments of amino-acid sequences.Gamier-Robson and Chou-Fasman methods were employed to analyze two-level structures of sequences.The Emini method was used for analyses of the accessibility of surface epitopes.Studies of hydrophilic proteins were undertaken using Kyte-Doolittle and Hopp-Woods methods.Analyses of antigen epitopes helped to reveal the antigen index using the Jameson-Wolf method.All sequences in the six strains of chlamydia micro virus capsid protein Vp3 were highly conserved,with the main differences being between Vp3 protein in Chp1 and the other five strains of the micro virus.The viral strain of Vp3 protein was based mainly on micro-alpha helix structures,and multiple epitopes were noted in highly conserved regions.Vp3 protein was highly conserved structurally,and was an important protein of the chlamydiaphage capsid.Vp3 protein has a complicated molecular structure,highly conserved regions with strong immunogenicity,and has considerable research value.

关 键 词：微病毒属 衣原体科 蛋白质类 生物信息学 结构 免疫 

分 类 号：R373.9[医药卫生—病原生物学]