Klotho基因G-395A多态性与慢性肾脏病患者动脉硬化的关系  

Association of G-395A Polymorphism of Human Klotho Gene with Arteriosclerosis in Patients of Chronic Kidney Disease

作　　者：陈金艳[1] 胡勇[1] 李慧[1] 赵玉 刘丽秋[3] 

机构地区：[1]青岛大学附属海慈医院肾病科,青岛266000 [2]山东省青岛市海慈医疗集团干保科,青岛266000 [3]青岛大学附属医院,青岛266000

出　　处：《中国中西医结合肾病杂志》2015年第6期517-519,共3页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:研究慢性肾脏病非透析患者Klotho基因启动子区域G-395A单核苷酸多态性的分布,探讨该基因多态性位点与慢性肾脏病动脉硬化的相关性。方法:116例慢性肾脏病非透析患者均进行彩超测定颈动脉内膜厚度,并记录动脉硬化的传统危险因素,根据测定结果分为动脉硬化组(56例)和对照组(60例)。应用Taq Man探针等位基因特异性杂交分析法对Klotho基因G-395A多态性位点进行分析。结果:G-395A多态性位点共检测出GG、GA、AA 3种基因型,频率分别为56.9%、32.8%和10.3%,符合Hardy-Weinberg平衡。动脉硬化组G-395A等位基因的频率显著低于对照组(35.1%vs49.0%,P=0.032)。logistic回归分析,调整传统危险因素后G-395A与动脉硬化呈负相关(β=-0.412,P=0.024)。结论:Klotho基因G-395A等位基因可能是慢性肾脏病动脉硬化的遗传学保护因素。Objective：To investigate the distribution of G - 395A polymorphism in promoter of Klotho gene in Chronic Kid-ney Disease Patients and analyze its association with arteriosclerosis. Methods：Genotyping of G - 395A polymorphism was performed in 56 arteriosclerotic patients of CKD and 60 controls using an allelic discrimination assay with TaqMan probes. Carotid artery intima- media thickness and atherosclerotic plaques were measured as a marker of atherosclerotic vascular damage. Results：Genotype fre-quencies of GG,GA and AA were 56. 9% . 32. 8% and10. 3% respectively. The distribution was in accordance with Hardy - Weinberg equilibrium. The frequency of the A allele carriers was signif - icantly lower in the arteriosclerosis group than in the control group （35. 1% vs 49. 0% ,P ﹤ 0. 05）. Logistic regression analysis revealed that after adjusted for traditional risk factors,the Aallele was＆amp;nbsp;negatively correlated with arteriosclerosis（β = - 0. 412,P ﹤ 0. 05）. Conclusion：The A allele of G - 395A polymorphism of Klotho gene may be a protective factor against arteriosclerosis in CKD patients.

关 键 词：慢性肾脏病 动脉硬化 多态性 单核苷酸 启动区(遗传学) 

分 类 号：R692[医药卫生—泌尿科学]