哮喘小鼠Rac1表达水平的改变及其与nuocytes极化的关系  

Changes of Rac1 expression levels and its relationship with nuocytes polarization in the asthmatic mouse model

作　　者：徐芸芸[1] 张梦莹[1] 吴静[1] 张盼[1] 彭静静[1] 张淡宜 齐晨[1] 纪晓昀[1] 苏兆亮[1] 王胜军[1] 许化溪[1] 

机构地区：[1]江苏大学医学院免疫学和免疫学检验系,镇江212013

出　　处：《免疫学杂志》2015年第7期576-579,共4页Immunological Journal

基　　金：国家自然科学基金面上项目(31270947);江苏省2012年度普通高校研究生科研创新计划(省立校助)(CXLX12_0677);江苏大学大学生科研立项(13A242)

摘　　要：目的检测哮喘小鼠呼吸道组织Rac1的表达水平,分析其与nuocytes相关细胞因子表达谱改变的关系,以探讨Rac1调节免疫应答类型和参与哮喘的可能机制。方法建立小鼠哮喘模型,以肺组织、外周血单个核细胞、肺泡灌洗液等为检材,应用实时荧光定量PCR方法分别检测Rac1、IL-5、IL-13、IL-33的mRNA水平;ELISA法检测血清nuocytes相关细胞因子水平。结果哮喘小鼠各组织IL-5、IL-13、IL-33的mRNA水平明显高于对照小鼠,而Rac1表达水平则明显降低,且相关性分析结果显示,气道Rac1的表达水平与IL-5、IL-13、IL-33的表达水平呈负相关。结论高水平表达nuocytes相关因子的哮喘小鼠,伴有呼吸道组织的Rac1表达水平明显降低的趋势,为进一步研究Rac1的免疫调节作用及其与哮喘发生发展的关系奠定了良好的基础。To explore the type and mechanism of Rac 1 immune response, the change of Rac 1 expression and its relationship with nuocytes-associated cytokines, such as IL-5, IL-13 and IL-33, were evaluated in asthmatic mice. The asthmatic mouse model was established and the lung tissue, peripheral blood mononuclear cells （PBMCs） and bronchoalveolar lavage （BAL） fluid were respectively obtained; the mRNA expression levels of Rac 1, IL-5, IL- l3 and IL-33 were determined by quantitative real-time PCR （qRT-PCR）. In addition, ELISA was used to detect the levels of nuocytes-associated cytokines in serum. The results showed that the expression levels of IL-5, IL-13 and IL-33 were increased in asthma mouse, while the Racl level in asthma mouse was significantly lower than that in control group. Taken together, there is a significant negative correlation between the expression levels of nuocytes-associated cytokines and Racl, suggesting the Racl level is significantly decreased in asthma model, accompanying with elevation of nuocytes-related factors.

关 键 词：RAC1 Nuocytes IL-5 IL-13 IL-33 

分 类 号：R562.25[医药卫生—呼吸系统]