不明原因反复性流产患者血清中sPD-1和sPD-L1与相关细胞因子的水平变化  

The change of soluble PD-1/PD-L1 and related cytokines in patients with unexplained recurrent abortion

作　　者：吴梦茹[1] 朱玥洁[1] 赵静[1] 张佳[2] 龚巧巧[2] 腊晓琳[1] 丁剑冰[2] 

机构地区：[1]新疆医科大学第一附属医院生殖助孕中心,乌鲁木齐830011 [2]新疆医科大学第一附属医院基础医学院,乌鲁木齐830011

出　　处：《免疫学杂志》2015年第7期603-607,共5页Immunological Journal

基　　金：自治区自然科学基金(2013211A087);国家自然科学基金(81160200);新疆自治区高校学科建设项目(XYDXK50780328)

摘　　要：目的探讨可溶性程序性死亡分子1(sPD-1)及其配体可溶性程序性死亡分子L1(sPD-L1)、相关细胞因子TGF-β、IL-10在不明原因反复性流产中的作用和相关性。方法收集不明原因反复性流产组、正常妊娠组和健康对照组血清,通过酶联免疫吸附试验(ELISA)方法检测sPD-1、sPD-L1的表达水平;流式液相多重蛋白定量技术(CBA)检测相关细胞因子TGF-β、IL-10水平变化。分析sPD-1/sPD-L1与TGF-β、IL-10相关性及其不明原因反复性流产的作用。结果在不明原因反复性流产组的血清中,sPD-1的表达水平明显高于正常妊娠组(P<0.01),sPD-L1在不明原因反复性流产组中的表达略高于正常妊娠组(P<0.01),且sPD-1/sPD-L1的比值在不明原因反复性流产组中明显升高。TGF-β、IL-10水平在不明原因反复性流产组中降低(P<0.01),而且sPD-1/sPD-L1的变化与TGF-β、IL-10呈负相关。结论不明原因反复性流产组中sPD-1的水平明显增多,Treg细胞相关细胞因子减少,可能不利于妊娠,易引发流产。To investigate the roles of soluble molecule programmed death 1 （sPD1） and soluble programmed death ligand L1 （sPD-L1）, as well as related eytokines TGF-β, IL-10 in unexplained recurrent abortion, we detected sPD-1/sPD-L1 levels by ELISA and analyzed TGF-13, IL-10 levels in sera of unexplained recurrent spontaneous abortion （USA） group, normal pregnancy group and the healthy control group patients by CBA assay. Result demonstrated that concentrations of sPD-1 and sPD-L1 in sera of URSA group and normal pregnancy group were increased, and URSA group was significantly higher than normal pregnancy group （P〈 0.01 ）. TGF-β and IL- 10 levels in URSA group and normal pregnancy group were decreased, as compared with healthy control group, and URSA group was significantly higher than normal pregnancy group （P〈 0.01 ）. In conclusion, the level of sPD-1 is increased obviously in URSA, and the related cytokines of Treg are significantly decreased; the change of sPD-1 is negatively correlated with the levels of TGF-I3 and IL-10, and may be involved in pregnancy. High level of sPD-1 may play important immunoregulatory role in the development of the pregnancy.

关 键 词：SPD-1 sPD-L1 不明原因反复性流产 细胞因子 

分 类 号：R714.21[医药卫生—妇产科学]