糖基化终末产物及其受体在糖尿病大鼠胃组织中的分布  

Distribution of advanced glycation end products and their receptor in the stomach of diabetic rats

作　　者：田佳星[1,2] 赵静波 李敏[1] 李君玲[2] 曹洋[2] Hans Gregersen 仝小林[1] 

机构地区：[1]中国中医科学院广安门医院内分泌科,北京市100053 [2]北京中医药大学研究生院,北京市100029 [3]丹麦奥尔胡斯大学临床医学研究所 [4]重庆大学生物医学工程学院,重庆市400044

出　　处：《世界华人消化杂志》2015年第17期2714-2721,共8页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81173259~~

摘　　要：目的:探讨了糖基化终末产物(advanced glycation end products,AGE)及其受体(receptor for advanced glycation end products,RAGE)在糖尿病大鼠胃组织中的分布.方法:糖尿病模型组与正常组大鼠饲养8w k,测量空腹血糖、糖化血清蛋白、胃壁组织学,免疫组织化学检测AGE与RAGE在胃壁组织的表达.结果:模型组大鼠胃黏膜层(781.47μm±137.82μm vs 709.85μm±169.41μm)和黏膜下层(233.39μm±134.05μm vs109.32μm±44.43μm)的厚度较正常组显著增加(P<0.05);AGE与RAGE在模型组大鼠胃组织的黏膜层(5.66±1.90 vs 2.25±0.52,2.79±0.54 vs 1.70±0.30)和肌层(37.37±7.38 vs 24.32±4.02,4.26±0.80vs 3.59±0.37)的分布较正常组显著增加(P<0.05).结论:AGE与RAGE在糖尿病大鼠的胃组织中表达上调,该异常分布可能与糖尿病胃肠功能障碍有关.AIM：To observe the distribution of advanced glycation end products（AGEs） and their receptor（RAGE） in the stomach of diabetic rats.METHODS：Diabetes mellitus（DM） and control（CON） rats were reared for eight weeks.Fasting plasma glucose（FPG）,glycated serum protein（GSP） and gastric layer thickness were measured.The expression of AGEs and RAGE was detected by immunohistochemical staining.RESULTS：The thickness of the mucosa（781.47 μm ± 137.82 μm vs 709.85 μm ± 169.41 μm） and submucosa（233.39 μm ± 134.05 μm vs 109.32 μm ± 44.43 μm） increased significantly in the DM group compared with the CON group（P〈0.05）.The expression of AGEs and RAGE in the mucosa（5.66 ± 1.90 vs 2.25 ± 0.52,2.79 ± 0.54 vs 1.70 ± 0.30） and muscle（37.37 ± 7.38 vs 24.32 ± 4.02,4.26 ± 0.80 vs 3.59 ± 0.37） layers of the stomach was significantly higher in the DM group than in the CON group（P〈0.05）.CONCLUSION：The expression of AGEs and RAGE is up-regulated in the stomach of diabetic rats.The increased levels of AGE and RAGE in gastric tissue may contribute to diabetic gastrointestinal dysfunction.

关 键 词：糖尿病 胃 糖基化终末产物 糖基化终末产物受体 

分 类 号：R587.2[医药卫生—内分泌]