高氧液对油酸性急性肺损伤家兔肺组织改变和肺表面活性物质的影响  

Effects of hyperoxia solution on pulmonary surfactant and histological changes in rabbits with oleic acid-induced acute lung injury

作　　者：陈亚丽[1] 周丹丹 程红霞[1] 张义长[1] 

机构地区：[1]西安交通大学医学部第一附属医院麻醉科,陕西西安710061 [2]西北妇女儿童医院麻醉科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2015年第4期496-500,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关计划项目(No.2004K12-G11)~~

摘　　要：目的：观察高氧液对急性油酸性肺损伤家兔肺组织形态改变及肺表面活性物质的影响。方法健康家兔30只,雌雄不拘,体质量2.0~2.5 kg,随机分为3组：空白对照组（n=10）；高氧液治疗组（n=10）；盐水对照组（n=10）。3组动物给予相应处理后分别在 ALI 模型制备前（基础值）、静脉输液前（损伤后）、输液后30 min、1 h、2 h 时观察动脉血气变化,2 h 后处死动物取肺组织做病理切片观察肺组织形态学改变和免疫组化检测肺表面活性物质的变化。结果高氧液治疗组在输注高氧液后2 h PaO2明显高于盐水对照组（P 〈0.001）,而 PaCO2则低于盐水对照组（P 〈0.001）。光镜下高氧液治疗组家兔肺组织损伤程度较盐水对照组轻。免疫组化及肺泡灌洗液测定结果显示,高氧液治疗组肺表面活性物质 A 含量明显高于盐水对照组（P 〈0.001）。结论静脉输注高氧液可减轻肺组织损伤程度,减少肺泡表面活性物质的破坏。Objective To observe the effects of hyperoxia solution on pulmonary surfactant and histology of rabbits with oleic acid-induced acute lung injury.Methods Thirty healthy rabbits weighing 2.0 - 2.5 kg were randomly divided into three groups with 10 rabbits each：control group,hyperoxia solution treatment group,and saline treatment group.Blood samples were taken for blood-gas analysis before and at 30,60 and 120 min after oleic acid or normal saline administration.Two hours later,the animals were killed and pathologic changes of lung tissue were observed microscopically.The expression of SP-A was investigated using the immunohistochemical method and image analysis system.Results PaO2 was significantly higher in hyperoxia solution treatment group than in saline treatment group two hours after treatment （P 〈 0.001 ）,but PaCO2 was significantly lower than that in saline treatment group （P 〈0.001）.Optical microscopy showed that lung tissue damage was milder in hyperoxia solution treatment group than in saline treatment group.The content of surfactant A in hyperoxia solution treatment group was significantly greater than that in saline treatment group （P 〈 0.001 ）.Conclusion Hyperoxia solution can lessen lung tissue injury and damage to pulmonary surfactant.

关 键 词：高氧液 油酸性急性肺损伤 肺表面活性物质 表面蛋白A 

分 类 号：R655.3[医药卫生—外科学]