氧自由基在急性单核细胞白血病发病中的作用  

The role of oxygen free radicals in the pathogenesis of acute mononuclear leukemia

作　　者：白改改[1] 路晨阳 申秋菊[1] 蒙珊[1] 惠凌云[2] 苏丹[1] 张王刚[1] 周芙玲[1] 

机构地区：[1]西安交通大学医学部第二附属医院血液内科,陕西西安710004 [2]西安交通大学医学部第一附属医院检验科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2015年第4期501-504,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81270597);2011年中央高校基本科研业务费专项资金资助项目(No.0602-08143041)~~

摘　　要：目的通过检测氧化应激的相关指标来探讨氧化应激参与急性单核细胞白血病(M5)发病及复发的机制。方法检测原发、复发、化疗缓解后M5患者细胞内活性氧的水平、外周血血浆中抗氧化酶活性、氧化损伤产物含量,并用透射电镜观察其外周血单个核细胞线粒体的超微结构。结果与正常对照组比较,原发组及复发组M5患者细胞内活性氧的平均荧光强度均显著增高(P<0.05);原发组M5患者血浆中抗氧化酶T-AOC、SOD活性降低,氧化损伤产物LDH、MDA、8-OHdG含量升高,复发组T-AOC、SOD活性显著下降,LDH、MDA、8-OHdG含量显著升高(P<0.05),而化疗缓解组T-AOC、SOD活性升高,LDH、MDA、8-OHdG含量降低;原发组M5患者线粒体轻度肿胀、嵴间隙增宽;复发组M5患者线粒体肿胀、畸形明显。结论氧化应激是M5发病的始动因素。线粒体是生物氧化进行的主要场所,活性氧及其相关的抗氧化酶之间动态平衡的破坏可能是促进复发的主要原因。Objective To study the mechanism of oxidative stress involved in the pathogenesis and relapse of acute monocytic leukemia （M5 ）.Methods We detected reactive oxide species （ROS）levels,conducted plasma analysis obtained from 76 M5 patients at diagnosis and at relapse,and observed the ultrastructure of mitochondria of mononuclear cells in peripheral blood by transmission electron microscope.Results Compared with that in the control group,the average fluorescence intensity of intracellular ROS was significantly increased in M5 groups, especially in the relapse patients （P 〈 0.05 ）.Low total antioxidative capacity （T-AOC）and antioxidant enzyme activity were characteristic of M5 at both diagnosis and relapse. However, lactate dehydrogenase （LDH ）, malondialdehyde （MDA）and 8-hydroxy-2’-deoxyguanine （8-OHdG）increased significantly at both diagnosis and relapse （P 〈 0.05 ）.Prominent ultrastructural abnormalities （mitochondrial swelling,outer membrane blebs,and aberrant cristae disorder）were present in patients with primary M5,and they were obviously abnormal in relapsing M5 patients.Conclusion Oxidative stress is the initiating factor of M5.Mitochondria are the main intracellular location for ROS generation.To maintain the dynamic balance between ROS and antioxidant defence may be the critical factor for preventing relapse.

关 键 词：急性单核细胞白血病(M5) 复发 活性氧自由基 线粒体 氧化应激 抗氧化酶 

分 类 号：R557.2[医药卫生—血液循环系统疾病]