白花蛇舌草注射液逆转人肝癌细胞BEL-7402/5-FU的多药耐药作用  

Effect of Hedyotic diffusainjection in reversing multi-drug resistance of human hepatoma BEL-7402/5-FU cells

作　　者：廖作庄[1] 韦丽美[2] 徐灵源[1] 梁钢[3] 

机构地区：[1]右江民族医学院附属医院药剂科,广西百色533000 [2]百色市人民医院人事科,广西百色533000 [3]广西医科大学药学院药理学教研室,广西南宁530021

出　　处：《西安交通大学学报（医学版）》2015年第4期554-557,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81160532)~~

摘　　要：目的研究白花蛇舌草注射液对BEL-7402/5-FU细胞多药耐药的逆转作用,并探讨其作用机制。方法四甲基偶氮唑蓝(MTT)法检测药物对BEL7402/5-FU细胞的半数抑制浓度(IC50);流式细胞仪检测细胞凋亡;ELISA双抗体一步夹心法测定酪氨酸激酶含量。结果无毒剂量的白花蛇舌草注射液(6μL/mL)联合5-FU对BEL-7402/5-FU细胞的IC50为636.5μmol/L,比单独应用5-FU的IC50(1 071.6μmol/L)明显下降,逆转倍数为1.68;加白花蛇舌草注射液后,5-FU对BEL-7402/5-FU细胞的凋亡比例明显增加,且显著下调BEL-7402/5-FU细胞的酪氨酸激酶含量。结论白花蛇舌草注射液对BEL-7402/5-FU细胞的多药耐药性具有一定的逆转作用,其机制可能与下调酪氨酸激酶有关。Objective To study the effect and mechanism of Hedyotic diffusa injection in reversing multi-drug resistance of BEL-7402/5-FU cells.Methods The IC50 of BEL-7402/5-FU cells was examined by MTT,apoptosis was detected by flow cytometry,and the content of tyrosine kinase in total protein of tumor cells was detected by enzyme-linked immunosorbent assay （ELISA）.Results The IC50 of Hedyotic diffusa injection （6 μL/mL ） combined with 5-FU （636.5 μmol/L）decreased significantly compared with that of 5-FU applied alone （1 071.6 6μmol/L）. The reversal on BEL-7402/5-FU was 1.68-fold.After injection of 5-FU plus Hedyotic diffusa ,the apoptosis rate of BEL-7402/5-FU cells was increased significantly and the tyrosine kinases of BEL-7402/5-FU cells decreased significantly.Conclusion Hedyotic diffusa injection plays a certain role in reversing multi-drug resistance of BEL-7402/5-FU cells,which may be related to decreasing their tyrosine kinases.

关 键 词：白花蛇舌草 肝癌细胞 多药耐药 凋亡 酪氨酸激酶 

分 类 号：R285.5[医药卫生—中药学]