不同浓度异丙酚对离体兔心肌动作电位的影响  

Effects of Different Concentrations of Propofol on Ventricular Action Potential in Isolated Rabbit Heart

作　　者：董丽[1] 高鸿[1] 刘艳秋[1] 安丽[1] 任益民[1] 胡柏龙 

机构地区：[1]贵州医科大学附院麻醉科,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第7期680-682,共3页Journal of Guiyang Medical College

基　　金：贵州省科技厅联合基金项目(黔科合LG字2011015号)

摘　　要：目的：观察不同浓度异丙酚对家兔心肌细胞动作电位时程的影响。方法：成年家兔24只,制备Langendorff离体心脏灌注模型,K-H液灌注10 min后随机均分为对照组（C组）、低浓度异丙酚组（LP组）及高浓度异丙酚组（HP组）,C组继续灌注K-H液60 min,LP和HP组分别灌注含4 mg/L与8 mg/L异丙酚的K-H液60 min;记录3组家兔平衡灌注10 min（T0）、继续灌注5 min（T1）、30 min（T2）、60 min（T3）时右心室前壁三层心肌动作电位,计算单相动作电位复极20%、50%、90%的时程（MAPD20、MAPD50、MAPD90）。结果：与T0比较,LP组和HP组T1~T3时MAPD20、MAPD50、MAPD90延长（P〈0.05）;与T1比较,两组T2~T3时MAPD20、MAPD50、MAPD90延长（P〈0.05）;与T2比较,两组T3时MAPD20、MAPD50、MAPD90延长（P〈0.05）;同一时点与C组和LP组比较,HP组T1~T3时MAPD20、MAPD50、MAPD90延长（P〈0.05）。结论：丙泊酚灌注时间延长和高浓度丙泊酚灌注,可延长心肌细胞动作电位的复极过程,这可能是临床使用丙泊酚导致心律失常的机制之一。Objective： To observe the effect of propofol on monophasic action potential duration （MAPD） of three - layer myocardium in isolated rabbit hearts. Methods： Twenty - four adult rabbits＇ isolated hearts were used to prepare Langendorff perfusion model. The hearts were randomly divided in- to 3 groups after perfusion with Krebs -Hensleit solution for 10 min： control group（ group C） ,low con- centration propofol group （group LP） and high concentration propofol group （group HP）. Groups LP and HP were perfused with 4 mg/L and 8 mg/L of propofol Krebs - Hensleit solution for 60 min, re- spectively. Group C continued perfusion of Krebs - Hensleit solution for 60 rain. The monophasic ac- tion potential of three - layer myocardium of right ventricular anterior wall was recorded at following time points： 10 min after balanced perfusion for （To） , 5 rain after continued perfusion（ T1 ） , 30 rain （T2） and 60 min （ T3 ）. The monophasic action potential duration of propofol at 20% （ MAPD2o ）, 50% （MAPDso）and 90% （MAPD9o） was calculated. Result： In groups LP and HP, MAPD2o, MAPDs0and MAPDgo at T2 ~ T3 were prolonged compared with their counterparts at TO （ P 〈 0.05 ）. In groups LP and HP, MAPD20, MAPD5o and MAPD9o at T2 - T3 were prolonged compared with their counterparts at T1 （ P 〈 0.05 ）. In groups LP and HP, MAPD20, MAPDs0 and MAPDgo at T3 were pro- longed compared with their counterparts at T2 （P 〈 0.05 ）. Compared with group C and Group LP, at the same time points in Group HP MAPD20, MAPDsoand MAPDg0were prolonged （P 〈0.05）. Conclu- sion： Prolonged propofol perfusion and high -dose propofol perfusion can prolong process of repolariza- tion of monophasic action potential of myocardium cell, which may result in arrhythmias.

关 键 词：丙泊酚 单相动作电位时程 电生理学技术 家兔 

分 类 号：R33-33[医药卫生—人体生理学] R614[医药卫生—基础医学]