子宫内膜腺癌中EMT的发生及miR200a/ZEB1信号通路在该过程中发挥的作用  

Occurrence of epithelia-mesenchymal transition in endometrial adenocarcinoma and the roles of miR200a/ZEB1 signaling pathway in this process

作　　者：张晓晖[1] 颜磊[1] 齐莎莎[1] 路真真 李明江[1] 赵兴波[1] 

机构地区：[1]山东大学附属省立医院妇产科,山东济南250021

出　　处：《山东大学学报（医学版）》2015年第7期48-52,57,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81272858;81202507)

摘　　要：目的检测上皮-间质转化(EMT)在子宫内膜腺癌中的发生,并探讨mi R200a/E盒结合锌指蛋白1(ZEB1)信号通路在其中发挥的作用。方法 1分组:取自石蜡切片的子宫内膜腺癌为A组(n=45),正常子宫内膜为B组(n=22);取自手术中的子宫内膜腺癌标本为C组(n=34),正常子宫内膜标本为D组(n=15);2采用免疫组化法检测A、B两组间E钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)、ZEB1的表达;采用Western blotting法检测C、D两组间上述3种蛋白的表达;3 Real-time RT-PCR法检测C、D两组间各蛋白相应m RNA及mi R200a的表达。结果 1 B组与A组比较,E-cadherin表达降低(P<0.05),Vimentin及ZEB1表达升高(P<0.05);2 D组与C组比较,E-cadherin蛋白及m RNA表达降低(P<0.05),Vimentin蛋白及m RNA表达升高(P<0.05),ZEB1蛋白及m RNA表达升高(P<0.05);3与C组数据相比,D组中mi R200a表达量降低(P<0.05),D组中mi R200a与E-cadherin的m RNA表达量之间存在正相关关系(r=0.63,P<0.01)。结论子宫内膜腺癌中明确发生了EMT过程,而mi R200a/ZEB1信号通路可对EMT过程中重要的分子标记物产生抑制作用。Objective To investigate the occurrence of epithelia-mesenchymal transition（ EM T） in endometrial carcinoma and examine the roles of mi R200 a / zinc finger E-box-binding protein 1（ ZEB1） signaling pathway in this process.Methods Different groups of samples were collected,including paraffin sections of endometrial adenocarcinoma tissues（ group A,n = 22）,endometrial tissues after resection of uterine myoma（ group B,n = 45）,endometrial adenocarcinoma samples obtained during operation（ group C,n = 15） and healthy endometrial tissues（ group D,n = 34）.Expressions of E-cadherin,Vimentin and ZEB1 in group A and group B were investigated with immunohistochemistry.Expressions of E-cadherin,Vimentin and ZEB1 and their m RNA in group C and group D were evaluated with Western blotting and Real-time RT-PCR. Expression of mi R200 a in group C and D was determined with Real-time RT-PCR.Results 1 Compared with group A,group B had significantly decreased expression of E-cadherin,and markedly increased expressions of Vimentin and ZEB1（ both P〈0. 05）. 2 Compared with group C,group D had decreased E-cadherin and its m RNA expressions but increased expressions of Vimentin and its m RNA,and ZEB1 and its m RNA（ all P〈0. 05）. 3 Compared with group C,group D exhibited remarkably elevated mi R200 a,which was positively correlated to E-cadherins m RNA（ r = 0. 63,P〈0. 01） as indicated by a linear regression analysis. Conclusions EM T occurs in endometrial adenocarcinoma. The mi R200 / ZEB1 signaling pathway can inhibit the important molecular markers in EM T.

关 键 词：上皮-间质转化 子宫内膜腺癌 微小RNA200a E盒结合锌指蛋白1 调控作用 

分 类 号：R711.74[医药卫生—妇产科学]