自身免疫性慢性荨麻疹患者外周血单个核细胞TLR2的表达及意义  

The Expression and Significance of TLR2 in Peripheral Blood Mononuclear Cells of Patients with Autoimmune Chronic Urticaria

作　　者：刘军连[1] 徐冰心[2] 王晓菲[2] 杜海平[1] 刘建军[1] 刘志国[2] 司少艳[2] 

机构地区：[1]解放军第306医院皮肤科,北京100101 [2]解放军第306医院特种医学实验研究中心,北京100101

出　　处：《中国皮肤性病学杂志》2015年第7期681-683,758,共4页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(30872284;81071402)

摘　　要：目的检测自身免疫性慢性荨麻疹(AIU)患者外周血单个核细胞(PBMCs)中Toll样受体2(TLR2)mRNA与蛋白的表达,探讨AIU的发病机制。方法采集AIU患者30例与正常对照组30例外周血,淋巴细胞分离液分离PBMCs,提取RNA,采用实时荧光定量逆转录PCR检测TLR2 mRNA表达;采用免疫荧光染色、流式细胞仪检测PBMCs中TLR2蛋白的表达。结果与正常对照组相比,AIU患者PBMCs中TLR2 mRNA的表达差异无统计学意义(P>0.05);蛋白表达增强,差异有统计学意义(P<0.05)。结论 AIU患者PBMCs中TLR2的表达增强,TLR2可能在AIU的发病中起作用。Objective To investigate the expression of TLR2 mRNA and protein in peripheral blood mononuclear ceils （PBMCs） of patients with autoimmune chronic urticaria （AIU） and to explore the immunology mechanism of AIU. Methods PBMCs from 30 patients with AIU and 30 normal controls were separated by Ficoll- Hypaque Solution. The expression of TLR2 mRNA and protein were detected respectively by real-time quan- titative reverse transcription polymerase chain reaction and by flow-cytometric analysis. Results The Results showed that, as compared to normal controls, the expression of TLR2 protein in PBMCs of patients with AIU significantly increased （ P 〈 0. 05 ）, but there was no difference （P 〉 0. 05 ） in the expression of TLR2 mRNA. Conclusion The expression of TLR2 increases in patients with AIU, and the findings suggest that TLR2 may vlav roles in the vathozenesis of AIU.

关 键 词：慢性荨麻疹 TOLL样受体2 自身免疫性慢性荨麻疹 表达 

分 类 号：R758.24[医药卫生—皮肤病学与性病学] R593.2[医药卫生—临床医学]