大骨节病患者血液红细胞介电响应性观察  

Dielectric response of erythrocyte in patients with Kaschin-Beck disease

作　　者：王斌[1] 陈慧英[2] 刘立亚[1] 

机构地区：[1]中央民族大学生命与环境科学学院生物科学系,北京100081 [2]中央民族大学生命与环境科学学院化学系,北京100081

出　　处：《中华地方病学杂志》2015年第7期481-484,共4页Chinese Journal of Endemiology

基　　金：中央民族大学“985工程”项目（MUC985-9）

摘　　要：目的测定大骨节病患者血液红细胞介电响应性变化规律，探讨大骨节病的发病机制，寻找大骨节病早期诊断及防治效果评估的更有效手段。方法根据介电泳原理和细胞介电响应现象，制备介电泳池并建立介电泳检测分离系统。在四川阿坝县大骨节病病区收集大骨节病患者（438例），同时在阿坝县大骨节病病区及非病区选取健康人（480例），采集大骨节病患者和健康人抗凝全血，分离红细胞，配制成浓度为105个／ml细胞悬液。加细胞悬液200μl于介电泳池中，测定红细胞的介电响应率。结果病区大骨节病患者与病区和非病区健康人群红细胞介电响应率比较差异有统计学意义伊=1．843，P均〈0．01）。其中大骨节病患者的红细胞介电响应率[（75．87士5．89）％]低于病区和非病区健康人群[（92．43±4．45）％、（92．81±5．01）％，P均〈0．01]；成年大骨节病患者的红细胞介电响应率[（69．57±6．87）％]低于儿童患者[（82．17±4．91）％，P〈0．01]。大骨节病不同患病程度患者的红细胞介电响应率比较差异有统计学意义（F=1．647，P〈0．01）。大骨节病患者的红细胞介电响应率与患病程度呈负相关（r=-0．87，P〈0．01），病情越重红细胞介电响应率越低。结论大骨节病患者有一定程度的红细胞病理性损伤，病情越重红细胞损伤越严重。红细胞介电响应性质的变化或许可以作为大骨节病患病程度判断和早期诊断的一项指标。Objective To measure the erythrocyte dielectric response variation of patients with KaschinBeck disease, to explore the pathogenesis of Kaschin-Beck disease and seek more effective means for evaluating the effectiveness of early diagnosis and control measures against Kaschin-Beck disease. Methods According to the principle of dielectrophoresis and cell dielectric response phenomena, dielectrephoretic pool was manufactured and dielectrophoretic detection and separation system was established. Patients with Kaschin-Beck disease （438） were selected in Kaschin-Beck disease areas of Aba County, Sichuan. At the same time, healthy persons （480） in KaschinBeck disease areas and non-Kaschin-Beck disease areas in Aba County were selected. Anticoagulant blood samples from patients with Kaschin-Beck disease and healthy persons were collected, erythrocytes were separated, cell suspension （10^5 cells/ml） was prepared and 200 μl cell suspension was added to the dielectrophoretic pool to measure erythrocyte dielectric response rate. Results Erythrocyte dielectric response rates were significantly different between patients living in Kaschin-Beck disease areas with Kaschin-Beck disease [（75.87 ± 5.89）%] and healthy persons living in Kaschin-Beck disease areas and non-Kaschin-Beck disease areas [（92.43 ± 4.45）%, （92.81 ±5.01）%, F = 1.843, P 〈 0.01）. Erythrocyte dielectric response rate was significantly reduced in patients with Kaschin-Beck disease compared with that of healthy persons （all P 〈 0.01）; erythrocyte dielectric response rate of adult patients with Kaschin-Beck disease was lower [（69.57 ± 6.87）%] than that of pediatric patients with Kaschin-Beck disease [（82.17 ± 4.91）%, P 〈 0.01]. Erythrocyte dielectric response rates were significantly different in patients with different degrees of Kaschin-Beek disease （F = 1.647, P 〈 0.01）. Erythrecyte dielectric response rate of patients with Kaschin-Beck disease was negatively correlated with prevalence of the disease （r =

关 键 词：大骨节病 介电泳 介电响应率 红细胞 

分 类 号：R684.1[医药卫生—骨科学]