亚慢性染砷对大鼠砷暴露标志物及脑组织总一氧化氮合酶活性的影响  

Effects of sub-chronic arsenic exposure on arsenic exposure markers and brain total NOS activity in rats

作　　者：王慧[1] 高彦辉[1] 孙洪娜[1] 曲丽莎[1] 姜丽欣[1] 孙殿军[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心卫生部病因流行病学重点实验室、黑龙江省普通高校病因流行病学重点实验室,150081

出　　处：《中华地方病学杂志》2015年第7期490-494,共5页Chinese Journal of Endemiology

基　　金：国家自然基金项目（81273013）

摘　　要：目的观察不同剂量饮水砷暴露后大鼠尿砷代谢形态，血、脑总砷含量及脑组织中总一氧化氮合酶（nitric oxide synthase，NOS）活性变化情况。方法将5周龄雄性SD大鼠按体质量采用随机数字表法分为4组，每组10只。对照组饮用蒸馏水，5mg／L亚砷酸钠（NaAa02）组饮用5m#L的NaAs02水溶液，10mg/LNaAs02组饮用10mg／L的NaAs02水溶液，50mr,／LNaAs02组饮用50mg／L的NaAs02水溶液。自由饮食、饮水。每周称重1次。连续染毒3个月后处死大鼠，收集血液、尿液、脑组织。采用高效液相色谱．氢化物发生．原子荧光光谱法检测尿中3价无机砷（3valence inorganicarsenic，iAs3＋）、5价无机砷（5valence inorganicarsenic，iAs5＋）、一甲基砷（monomethylated arsenic，MMA）和二甲基砷（dimethylated arsenic，DMA）的含量；氢化物原子荧光光度法检测血、脑总砷含量；分光光度计法检测血、脑组织中总NOS活性。结果①体质量：染砷第5～12周，50m#LNaAs02组大鼠体质量[（391．66土32．88）、（410．17±33．47）、（426．96±33．49）、（427．15．±32．20）、（441．78±33．69）、（438．27±33．05）、（440．98±33．33）、（441．46±32．45）g]与对照组[（420．93±21．13）、（441．52±28．85）、（462．45±30．57）、（470．16±31．17）、（484．92±32．93）、（483．79±29．63）、（482．02±29．14）、（483．89±29．31）g]比较明显降低（P均〈0．05）。②尿砷：对照组，5、10、50mg／LNaAs02组iAQ3＋含量中位数（0．00、57．30、236．33、857．80μg／L）比较，差异有统计学意义（X2=31．982，P〈0．01）；对照组，5、10、50mg／LNaAs02组iAs“含量中位数（0．00、0．00、80．75、162．90μg／L）比较，差异有统计学意义（X2=24．206，P〈0．01）；对照组，5、10、50mg／LNaAs02组DMA含量中位数（12．83、1711．13、10386．20、37038．90μg／L）比较，差异有统计学意Objective To observe the changes of the tote nitric oxide synthase （NOS） activity in brain tissue, the metabolism of arsenic speciations in urine and the totle contents in blood, brain after rats drinking water containing different doses of arsenic. Methods Forty SD rats were divided into 4 groups according to random number table, 10 rats in each group： control group, 5 mg/L NaAsO2 group, 10 mg/L NaAsO2 group and 50 mg/L NaAsO2 group. The animals were allowed free access to water and food. Body mass was weighted once a week.Expose to arsenic was continued for three months, then the animals were put to death and their blood, urine and brain tissues were collected. Determination of four kinds of speciations of arsenic （3 valence inorganic arsenic, iAs3＋; 5 valence inorganic arsenic, iAs5＋; monomethylated arsenic, MMA; dimethylated arsenic, DMA） in urine was carried out by high performance liquid chromatography-hydride atomic fluorescence spectrometry. Totalarsenic concentration in blood and brain tissue was detected by Atomic Fluorescence Spectrometry. The activity of total NOS in blood and brain tissue was detected using the spectrophotometer method. Results ①Weight： at the 5th - 12th week after arsenic exposure, compared with the weight of control group [（420.93 ± 21.13）, （441.52 ± 28.85）, （462.45 ± 30.57）, （470.16 ± 31.17）, （484.92± 32.93）, （483.79 ± 29.63）, （482.02 ± 29.14）, （483.89 ± 29.31） g], weight of rats in 50 mg/L NaAsO2 group [（391.66 ± 32.88）, （410.17 ±33.47）, （426.96 ±33.49）, （427.15 ± 32.20）, （441.78 ± 33.69）, （438.27 ±33.05）, （440.98 ±33.33）, （441.46 ± 32.45） g] was significantly lighter （all P 〈 0.05）. ② Urine arsenic： the medians of iAs3＋ content （0.00, 57.30, 236.33, 857.80 μg/L） were compared between control group, 5, 10 and 50 mg/L NaAsO2 groups, the differences were statistically significant （X2 = 31.982, P 〈 0.01）; the medians of iAs5＋ content （0.00, 0.00, 80.75, 162.90

关 键 词：砷 大鼠 脑 一氧化氮合酶 

分 类 号：R599.1[医药卫生—内科学]