纳米羟基磷灰石/聚羟基丁酸酯骨组织工程支架的制备及性能表征  

Fabrication and properties of nano-hydroxyapatite/poly(3-hydroxybutyrate) ultrafine-fibrous scaffolds for bone tissue engineering

作　　者：管东华[1] 林映荷[2] 黄建生[1] 陈治清[2] 

机构地区：[1]广东省口腔医院.南方医科大学附属口腔医院特诊中心,广东省广州市510280 [2]四川大学华西口腔医学院,四川省成都市610041

出　　处：《中国组织工程研究》2015年第25期3983-3989,共7页Chinese Journal of Tissue Engineering Research

基　　金：广东省医学科学技术研究基金资助(A2013104)~~

摘　　要：背景:高分子聚合物聚羟基丁酸酯具有优良的生物相容性、生物降解性及压电性,但也存在脆性大、亲水性较差等不足。目的:制备不同组成比例的电纺纳米羟基磷灰石/聚羟基丁酸酯纤维支架材料,分析其结构及性能。方法:通过气流-高压静电纺丝技术制备纳米羟基磷灰石质量百分比分别为0、10%、20%、30%的电纺纳米羟基磷灰石/聚羟基丁酸酯纤维支架,检测支架材料的微观结构、基团组成、晶相分布、热学性能及表面润湿性。结果与结论:扫描电镜观察显示,随着纳米羟基磷灰石含量的增大,越来越多的纳米羟基磷灰石颗粒分布于复合纤维表面且分布趋于均匀,到含量达到30%时,纳米羟基磷灰石已基本布满纤维表面,纤维表面的粗糙度也随之增加;差示扫描量热法及X射线衍射结果表明,纳米羟基磷灰石的加入可以降低复合纤维中聚羟基丁酸酯的结晶度及结晶规整程度,且纳米羟基磷灰石含量越大效果越明显;随着纳米羟基磷灰石含量的增大,复合支架表面的接触角逐渐降低,亲水性有所提高。表明将纳米羟基磷灰石与聚羟基丁酸酯复合进行电纺可以有效提高材料的表面润湿性及结晶度,改善材料的亲水性及脆性,且纳米羟基磷灰石含量越高作用越明显。BACKGROUND： Poly（3-hydroxybutyrate） is approved as its excellent biocompatibility, biodegradability and piezoelectric properties, but there are also some deficiencies, such as high breakability and poor hydrophilicity. METHODS： Poly（3-hydroxybutyrate） was mixed with different mass percentages of nanohydroxyapatite（0, 10%, 20% and 30%） to prepare new composite fibrous scaffolds through electrospinning process. The microstructure, group composition, crystalline phase distribution, thermal properties and surface wettability of the scaffolds were detected. RESULTS AND CONCLUSION： Under the scanning electron microscope, with the increase of nano-hydroxyapatite content, more and more nano-hydroxyapatite particles were distributed evenly on the composite fiber surface; the fiber surface was basically covered with nano-hydroxyapatite particles at the content of 30%, and the roughness of the fiber surface also increased. Results from differential scanning calorimetry andX-ray diffraction showed that the nano-hydroxyapatite reduced the crystallinity of poly（3-hydroxybutyrate） and the crystal tacticity, and this phenomenon became more evident with the increase of nano-hydroxyapatite content. Additionally, the higher the content of nano-hydroxyapatite content, the lower the contact angle and the higher the hydrophily. These findings indicate that the nano-hydroxyapatite/poly（3-hydroxybutyrate） ultrafine-fibrous scaffold using electrospinning technology can effectively improve the surface wettability and crystallinity of the material as well as the material hydrophily and brittleness, and the higher the content of nano-hydroxyapatite, the more obvious the effect.

关 键 词：生物材料 骨生物材料 高压静电纺丝 纳米羟基磷灰石 聚羟基丁酸酯 性能 

分 类 号：R318[医药卫生—生物医学工程]