筋膜成纤维细胞与纤维蛋白凝胶的体外生物相容性  

Biocompatibility in vitro between fascia fibroblasts and fibrin glue

作　　者：辛培成[1] 郭其勇[1] 李杰[2] 赵德伟[3] 杨圣[3] 

机构地区：[1]泰山医学院附属医院骨科,山东省泰安市271000 [2]泰山医学院附属医院血液内科,山东省泰安市271000 [3]大连大学附属中山医院骨科,辽宁省大连市116001

出　　处：《中国组织工程研究》2015年第25期4048-4053,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30870647-C100202)~~

摘　　要：背景:纤维蛋白凝胶是一种天然可降解的生物支架材料,具备可用于组织工程支架的共性,被越来越多地用做种子细胞载体应用于组织工程修复。目的:观察兔筋膜成纤维细胞与纤维蛋白凝胶的体外生物相容性。方法:采用组织块贴壁法培养新西兰大白兔皮下固有筋膜组织成纤维细胞,以胰酶消化法对其进行传代。将第4代成纤维细胞悬液与纤维蛋白凝胶共培养,以倒置相差显微镜动态观察纤维蛋白凝胶表面成纤维细胞的形态及增殖情况;共培养5 d时,以免疫荧光染色激光共聚焦显微镜鉴定成纤维细胞,扫描电镜观察成纤维细胞的生长贴附情况。结果与结论:倒置相差显微镜下显示,纤维蛋白凝胶表面的成纤维细胞形态与单纯培养成纤维细胞无明显差别;扫描电镜显示,成纤维细胞在纤维蛋白凝胶表面伸展充分,伸出的"伪足"与纤维蛋白凝胶有很好的贴附并分泌基质样物质,可见纤维蛋白凝胶并未改变成纤维细胞的形态学特征;激光共聚焦显微镜显示,成纤维细胞波形蛋白呈阳性表达,证明成纤维细胞种植在纤维蛋白凝胶表面后性质未发生变化,未被诱导分化。表明筋膜成纤维细胞与纤维蛋白凝胶在体外有很好的生物相容性。BACKGROUND： Fibrin glue is a natural biodegradable scaffold, which can be used for tissue-engineered scaffolds, and is increasingly used as seed cell carrier for tissue engineering repair. OBJECTIVE： To investigate the biocompatibility in vitro of rabbit fascia fibroblasts and fibrin glue. METHODS： Tissue explants adherent method was used to culture fibroblasts from subcutaneous deep fascia tissue of New Zealand white rabbits. The fibroblasts could be passaged with trypsin digestion method. Suspension of passage four fibroblasts was co-cultured with fibrin glue. Morphology and proliferation of fibroblasts on the surface of fibrin glue were dynamically observed under the inverted phase contrast microscope. At 5 days after co-culture, fibroblasts were identified by immunofluorescence staining under the laser scanning confocal microscope. The fibroblast growth and adhesion were observed under the scanning electron microscope. RESULTS AND CONCLUSION： There was no significant difference in fibroblast morphology between co-culturefibroblasts and pure culture fibroblasts with inverted phase contrast microscope. Scanning electron microscope demonstrated that fibroblasts fully extended in fibrin glue surface, and showed a good adhesion between the ＂pseudopodium＂ and fibrin glue, and secreted matrix material. It is clear that the fibrin glue did not alter the morphologic features of fibroblasts. Laser scanning confocal microscope revealed that fibroblasts were positive for vimentin. These verified that properties of fibroblasts did not change after they were seeded in fibrin glue surface and did not be induced to differentiate. There is a very good biocompatibility between fascia fibroblasts and fibrin glue in vitro.

关 键 词：生物材料 材料相容性 纤维蛋白凝胶 生物相容性 兔 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]