双向电泳在植物蛋白质组中的应用及影响因素分析  

Application of two-dimensional gel electrophoresis in plant proteomics and its influence factors analysis

作　　者：占志勇[1] 黄建建[1] 汪阳东[2] 田径[1] 幸伟年[1] 高伟[1] 龚春[1] 

机构地区：[1]江西省林业科学院,江西南昌330013 [2]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《南方林业科学》2015年第2期1-5,共5页South China Forestry Science

基　　金：江西省林业科学院重大项目"南方主要木本粮油植物种质资源收集与保存"部分内容

摘　　要：双向电泳(two-dimensional gel electrophoresis,2-DE)是蛋白质组学研究中常用的技术之一,因具有较高的稳定性和重复性,在植物蛋白质组学中应用广泛。蛋白质双向电泳(2-DE)过程复杂、时间持久以及影响因素众多,本文立足蛋白质双向电泳实验过程中可能出现的各种现象,如:分离的蛋白质点过少、条纹过多导致凝胶背景模糊、高丰度蛋白点聚集沉淀及低丰度蛋白点被掩盖等,着重分析其产生的原因并提出具体的解决办法,为蛋白质双向电泳技术初学者提供参考。The different proteins have the different isoelectric point and molecular weight, and because of this, two-dimensional gel electrophoresis （2-DE） can separate the proteins mixture effectively. After some years development, the stability and repeatability of 2-DE have been improved greatly and now it has become one of the main core technologies in the proteomics research, as well as it has a widely application in plant proteomics. Some characteristics of 2-DE, such as a complex process, the long time and so on, can lead it failed in the end. In the process of 2-DE, there are often some phenomenon can affect the experiments＆#39; results containing so few proteins separated in the gel, much more stripes resulted in a very bad resolution, the high expression proteins often gathered and precipitated, and the low expression proteins point were be covered. The causes for the above phenomenon were analyzed and the resolve methods were put forward, and it provided references for beginners learning the technology of protein 2-DE.

关 键 词：双向电泳 植物蛋白质组学 应用 

分 类 号：S794.3[农业科学—林木遗传育种] Q51[农业科学—林学]