沉水樟组织培养研究  

Studies on tissue culture of Cinnamomum micranthum

作　　者：罗坤水[1] 胡庆[2] 罗忠生[2] 林洪[1] 杨春霞[1] 

机构地区：[1]江西省林业科学院,江西南昌330013 [2]吉安市林业科学研究所,江西吉安343011

出　　处：《南方林业科学》2015年第2期12-14,27,共4页South China Forestry Science

基　　金：江西省科技支撑计划项目(项目编号:2009BNA05800);吉安市林业局项目"沉水樟扦插快繁及开发利用研究"

摘　　要：以沉水樟当年生半木质化枝条为材料,通过试验设计得到沉水樟最适诱导培养基为MS+6-BA 0.5 mg/L+NAA 0.2 mg/L(p H=6.0),最佳继代增殖培养基和生根培养基分别为1/2MS+6-BA 0.8 mg/L+NAA 0.05 mg/L+Na2S2O3150mg/L(p H=5.5)和1/2MS+IBA 1.0 mg/L+NAA 0.05 mg/L,增殖系数达到2.5,有效生根率达到96%,且在增殖培养基中添加一定浓度的Na2S2O3,解决了新抽芽顶梢或叶枯顶或掉叶难题,为沉水樟快繁提供了一种有效方法。One-year half-lignified shoots from Cinnamomum micranthum trees were used to study the optimum media for tissue culture. The results showed that optimum induction medium for C. micranthum is MS＋6-BA 0.5 mg/L＋NAA 0.2 mg/L （pH=6.0）. The best proliferation medium is 1/2MS＋6-BA 0.8 mg/L＋NAA 0.05 mg/L＋ Na2S2O3 150 mg/L （pH=5.5）, and its multiplication coefficient can reach 2.5. Rooting rate is above 96%in the optimum rooting medium that is 1/2MS＋IBA1.0 mg/L ＋NAA0.05 mg/L. Moreover, proliferation medium containing certain concentration Na2S2O3 can efficiently resolve the difficult problem that the shoot top withered up or leaves fallen during the process of the proliferation culture, and provide an effective method for rapid propagation of C. micranthum.

关 键 词：沉水樟 组织培养 

分 类 号：S792.23[农业科学—林木遗传育种] Q813.1[农业科学—林学]