Effect of electro-acupuncture on gene expression in heart of rats with stress-induced pre-hypertension based on gene chip technology  

作　　者：Guo Yan Xie Xiaojia Guo Changqing Wang Zhaoyang Liu Qingguo 

机构地区：[1]School of Acupuncture,Moxibustion and Tuina,Beijing University of Chinese Medicine

出　　处：《Journal of Traditional Chinese Medicine》2015年第3期285-294,共10页中医杂志（英文版）

基　　金：the National Natural Science Foundation of China(Based on the Systems Biology of Acupuncture on Irritable Early Hypertension Control Mechanism and Intervention Study Targets Research,No.81072861)

摘　　要：OBJECTIVE:To explore electro-acupuncture's(EA's)effect on gene expression in heart of rats with stress-induced pre-hypertension and try to reveal its biological mechanism based on gene chip technology.METHODS:Twenty-seven Wistar male rats were randomly divided into 3 groups.The stress-induced hypertensive rat model was prepared by electric foot-shocks combined with generated noise.Molding cycle lasted for 14 days and EA intervene was applied on rats in model + EA group during model preparation.Rat Gene 2.0 Sense Target Array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction(RT-PCR) method.RESULTS:Compared with blank control group,390 genes were changed in model group;compared with model control group,330 genes were changed in model + EA group.Significance analysis of gene function showed that the differentially expressed genes are those involved in biological process,molecular function and cellular components.RT-PCR result of the screened key genes is consistent with that of gene chip test.CONCLUTION:EA could significantly lower blood pressure of stress-induced pre-hypertension rats and affect its gene expression profile in heart.Genes that related to the contraction of vascular smooth muscle may be involved in EA's anti-hypertensive mechanism.OBJECTIVE：To explore electro-acupuncture＇s（EA＇s）effect on gene expression in heart of rats with stress-induced pre-hypertension and try to reveal its biological mechanism based on gene chip technology.METHODS：Twenty-seven Wistar male rats were randomly divided into 3 groups.The stress-induced hypertensive rat model was prepared by electric foot-shocks combined with generated noise.Molding cycle lasted for 14 days and EA intervene was applied on rats in model ＋ EA group during model preparation.Rat Gene 2.0 Sense Target Array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction（RT-PCR） method.RESULTS：Compared with blank control group,390 genes were changed in model group;compared with model control group,330 genes were changed in model ＋ EA group.Significance analysis of gene function showed that the differentially expressed genes are those involved in biological process,molecular function and cellular components.RT-PCR result of the screened key genes is consistent with that of gene chip test.CONCLUTION：EA could significantly lower blood pressure of stress-induced pre-hypertension rats and affect its gene expression profile in heart.Genes that related to the contraction of vascular smooth muscle may be involved in EA＇s anti-hypertensive mechanism.

关 键 词：STRESS PREHYPERTENSION ELECTROACUPUNCTURE Gene expression profiling HEART 

分 类 号：R245[医药卫生—针灸推拿学]