Effect of acupuncture at Fengchi(GB 20)on the activity of myosin light chain kinase in the middle meningeal artery of migraine modeled rats  

作　　者：Zhou Peijuan Wang Aicheng Li Bai Liu Chunyan Wang Yu 

机构地区：[1]Department of Acupuncture and Moxibustion,the Third Affiliated Hospital of Beijing University of Chinese Medicine [2]Department of Gastroenterology,Beijing University of Chinese Medicine,Dongzhimen Hospital East Area

出　　处：《Journal of Traditional Chinese Medicine》2015年第3期301-305,共5页中医杂志（英文版）

基　　金：the Beijing Natural Science Found Program[Fengchi point(GB 20)Controlling Mechanisn of MLCK Signal System of the Migraine,No.7092054

摘　　要：OBJECTIVE:To study the effect of acupuncture at Fengchi(GB 20) on the activation of myosin light chain kinase(MLCK) in the middle meningeal artery of migraine modeled rats.METHODS:Forty-four clean grade healthy female Sprague-Dawley(SD) rats were randomly divided into four groups:the control group,blank control group,Fengchi(GB 20) acupuncture group,and Fengchi(GB 20) prevention group.Neurogenic inflammation of these rats was induced by electrical stimulation.The γ-^(32)P infiltration method was then used to detect MLCK activation in the middle meningeal artery,and immunocytochemistry was applied to detect the structural protein expression of MLCK.RESULTS:The migraine model was successfully established in the rats.Compared with the control group,MLCK activation was significantly decreased in the blank control group(P < 0.01).CONCLUSION:The activation of MLCK in the middle meningeal artery was increased by acupuncture at Fengchi(GB 20),indicating its effectiveness in preventing and curing on acute migraine attacks.OBJECTIVE：To study the effect of acupuncture at Fengchi（GB 20） on the activation of myosin light chain kinase（MLCK） in the middle meningeal artery of migraine modeled rats.METHODS：Forty-four clean grade healthy female Sprague-Dawley（SD） rats were randomly divided into four groups：the control group,blank control group,Fengchi（GB 20） acupuncture group,and Fengchi（GB 20） prevention group.Neurogenic inflammation of these rats was induced by electrical stimulation.The γ-^32P infiltration method was then used to detect MLCK activation in the middle meningeal artery,and immunocytochemistry was applied to detect the structural protein expression of MLCK.RESULTS：The migraine model was successfully established in the rats.Compared with the control group,MLCK activation was significantly decreased in the blank control group（P 〈 0.01）.CONCLUSION：The activation of MLCK in the middle meningeal artery was increased by acupuncture at Fengchi（GB 20）,indicating its effectiveness in preventing and curing on acute migraine attacks.

关 键 词：Myosin-light-chain kinase Point GB 20（Fengchi） Migraine disorders Middle meningeal artery 

分 类 号：R245[医药卫生—针灸推拿学]