β1受体阻滞剂对脓毒症大鼠心肌损伤的影响  

Effects of β1 receptor blocker on myocardial injury in rats with sepsis

作　　者：李宛霞[1] 陶少宇[1] 

机构地区：[1]南昌大学第二附属医院急诊科,江西南昌330006

出　　处：《中国急救医学》2015年第7期577-579,共3页Chinese Journal of Critical Care Medicine

基　　金：江西省卫生计生委科技计划（20141068）

摘　　要：目的探讨β1受体阻滞剂对脓毒症大鼠心肌损伤的影响和机制。方法将健康雄性大鼠72只随机分为对照组、脓毒症组、治疗组，每组24只。脓毒症组采用盲肠结扎穿孔法（CLP）建立脓毒症模型，对照组仅剖腹而不进行CLP。脓毒症组和对照组在关腹后皮下注射生理盐水3mL／100g；治疗组除皮下补液外，经尾静脉注射艾司洛尔15mg／（kg·h）持续静脉泵入，分别于3、6、12、24h采集标本，在每个时间点每组大鼠均为6只，观察三组血清肿瘤坏死因子-α（TNF-α）、心肌组织核转录因子-κBp65（NF—κBp65）、心肌肌钙蛋白I（cTnI）的变化。结果与对照组比较，脓毒症组在各时间点血清TNF-α浓度、cTnI浓度及心肌组织NF—κBp65表达均显著升高（P〈0．05）；与脓毒症组比较，治疗组在各时间点血清TNF—α浓度、cTnI浓度及心肌组织NF—κBp65表达均降低（P〈0．05）。结论β1受体阻滞剂艾司洛尔可减轻脓毒症大鼠心肌损伤，其机制可能与抑制心肌NF—κBp65的表达，降低血清促炎介质的产生有关。β1受体阻滞剂能改善脓毒症失控的炎症反应以及保护心肌功能。Objective To investigate the effects and mechanisms of β1 receptor blocker on myocardial injury in rats with sepsis. Methods Seventy -two healthy male rats were randomly divided into control group, sepsis group, treatment group, with 24 rats in each group. Rat sepsis model was established by the method of cecal ligation and puncture （CLP）. Rats in control group were only performed aparotomy. Rats in sepsis group and control group were injected of saline （3 mL/100 g） while rats in treatment group were injected of esmolol [ 15 mg/（kg · h）] by micro pump. Samples were collected at 3th, 6th, 12th, 24th hour and there were 6 rats at each time point in each group. The changes of serum tumor necrosis factor -α （ TNF - α）, serum cardiac troponin I （ cTnI）, nuclear factor- κB p65 （NF - κB p65 ） of myocardial tissue and myocardial pathological morphology were observed. Results Compared with control group, the level of TNF - α, the concentration of cTnI and expression NF - κB p65 in myocardial tissue in sepsis group at each time point were significantly increased （ P 〈 0.05 ）. Compared with sepsis group, the level of TNF - α, the concentration of cTnI and expression NF- κB p65 in myocardial tissue in treatment group at each time point were decreased and the difference was statistically significant （P 〈 0.05 ）. Conclusionβ1 receptor blocker esmolol could reduce myocardial damage in septic rats. The mechanism may be related to inhibiting the expression of NF - κB p65 in myocardial tissue and reducing the production of serum pro - inflammatory mediators. β1 receptor blockers could improve the uncontrolled inflammatory response and protect myocardial function in sepsis.

关 键 词：Β1受体阻滞剂 艾司洛尔 脓毒症 心肌损伤 肿瘤坏死因子-α(TNF-α) 核转录因子-κB p65(NF—κB p65) 心肌肌钙蛋白I(cTnI) 

分 类 号：R972[医药卫生—药品]