不同细胞构筑在高压氧治疗迟发型脑病中的功能研究  

Role of different cell architectural in HBO therapy of delayed encephalopathy

作　　者：薛莉[1] 王文岚[1] 李娅[1] 任杰[1] 李金声[1] 常耀明[1] 谢小萍[1] 

机构地区：[1]第四军医大学航空航天医学系卫生学教研室,陕西西安710032

出　　处：《中国急救医学》2015年第7期651-656,I0003,I0004,共8页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金项目（81101466）

摘　　要：目的通过研究高压氧（HBO）治疗急性CO中毒大鼠皮层不同细胞构筑在神经细胞凋亡中的差别，探讨HBO治疗急性CO中毒的机制及靶向定位。方法利用雄性SD大鼠，建立急性CO中毒模型。应用免疫组织化学以及免疫荧光的方法，测定在CO中毒和HBO治疗1d、3d、7d、14d和21d后Caspase-3、NeuN和MMP-9的表达变化。结果急性CO中毒后，皮层神经细胞凋亡随中毒后时间延长而加重；不同细胞构筑发生凋亡程度不同，内颗粒层和内锥体细胞层最明显；Caspase-3与MMP-9在内颗粒层与内锥体细胞层变化最明显，在1d开始增多，3d达到最大值，7d开始减少；HBO治疗后，MMP-9和Caspase-3降低，尤其以内颗粒层与内锥体细胞层最明显；且HBO治疗7d后，降低最明显。结论急性CO中毒后，皮层各层神经细胞均发生凋亡现象，内颗粒层和内锥体细胞层最明显；凋亡可能与MMP-9降解神经细胞周围的基质，Caspase-3促进凋亡有关；HBO治疗可能是通过调控复杂的蛋白表达机制，调节MMP-9以及Caspase-3的表达水平，最终抑制神经细胞的凋亡；HBO治疗7d对凋亡的作用最明显。Objective To investigate the effects and mechanism on neuronal apoptosis of cortex different cell architectural after acute carbon monoxide （CO） poisoning and hyperbaric oxygen （HBO） therapy. Methods The animal model of acute CO poisoning was established. Then the SD rats were treated with hyperbaric oxygen therapy. Immunohistochemistry and were used to determine the expression level of Caspase -3, NeuN and MMP -9 at 1 d, 3 d, ？ d, 14 d and 21 d. Results Different cell of rat cortex involved in neuronal apoptosis after acute CO poisoning and HBO therapy; the internal - granular layer and internal - pyramidal layer of cortex most sensitivity in apoptosis after acute CO poisoning. Caspase -3 and MMP -9 regulates neuronal apoptosis： it began to increase at 1 d, reached the maximum at 3 d, started to decrease at 7 d; for the CO poisoning rats treated with HBO therapy, MMP- 9 and Caspase -3 in cortex were obviously decreased, especially in internal -granular layer and internal - pyramidal layer. On 7 day, the inhibitory effect of HBO therapy is the most obvious. Conclusion Neuronal apoptosis of cortex depend on different cell architectural, internal - granular layer and internal - pyramidal layer are most sensitive in the 6 layers. MMP - 9 expression may responsible on the apoptosis and may related with matrix degradation. Also, the expression of Caspase - 3 and MMP- 9 may related with the function of neurons. HBO therapy may control the expression of apoptosis related proteins and reduce the expression of MMP - 9 and Caspase - 3, then inhibit neuronal apoptosis.

关 键 词：高压氧治疗 急性一氧化碳中毒 基质金属蛋白酶-9 神经细胞凋亡 

分 类 号：R459.6[医药卫生—治疗学]