基于薄液膜蒸发的玻璃化冷冻方法中冷冻载体的传热优化  

Heat Transfer Optimization for Frozen Carrier in Vitrification Method Utilizing Thin Film Evaporation

作　　者：苏风民[1] 石珂玮 王朝冰 赵楠楠[1] 邓洋波[1] 马鸿斌[1] 

机构地区：[1]大连海事大学轮机热能工程研究所,大连116026

出　　处：《工程热物理学报》2015年第7期1510-1514,共5页Journal of Engineering Thermophysics

基　　金：国家自然科学基金(No.51376027);中央高校基本科研业务费专项资金(No.3132015211)

摘　　要：玻璃化冷冻方法是细胞超低温保存的有效冷冻方法之一。降温速率的提高有利于细胞内外溶液玻璃化程度的增加,同时可以降低低温保护剂的浓度,减少低温保护剂对细胞的毒性伤害和渗透性损伤。本文针对冷冻载体在基于薄液膜蒸发的超高速冷却过程中的传热过程进行了优化分析。研究结果显示:细胞悬浮液的传热热阻在总传热热阻中的比重最大,设法减低它是强化细胞冷冻传热过程的有效手段之一;另外液氮蒸发传热系数的提高在一定范围内可以强化冷冻传热过程,但强化效果会随着蒸发传热系数的增加而减小。上述研究成果为基于薄液膜蒸发的玻璃化冷冻新方法走向实用提供了技术保障。Vitrification is one of effective methods for cell cryopreservation. An ultra-fast cooling rate is beneficial to improve the vitrification level of the extra and intracellular solution and decrease the concentration of cryoprotectant. The relatively low concentration cryoprotectant can decrease the damaging toxic and/or osmotic effects on cell survival. This study tried to optimize the heat transfer of the frozen carrier which is used for the ultra-fast cooling method utilizing the thin film evaporation. The results showed that the share of the heat resistance of the cell suspension is most in the total heat resistance of the frozen carrier. Reducing it is one of the effective methods enhancing heat transfer in the cooling process of the cells. Besides, improving the evaporation heat transfer eoefficient of the liquid nitrogen can enhance the whole heat transfer process, but the enhancing effect decreases with the evaporation heat transfer coefficient increasing. The current inves- tigation will support better the development of the vitrification method utilizing thin film evaporation.

关 键 词：细胞冷冻 传热优化 冷冻载体 

分 类 号：TK124[动力工程及工程热物理—工程热物理]